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Invitrogen
Phospho-VAV2 (Tyr172) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-VAV2 (Tyr172) Antibody (PA5-143788) in ICC/IF
Immunocytochemistry analysis of Phospho-VAV2 (Tyr172) in control and pervanadate-treated human A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40. Then labeled with rabbit Phospho-VAV2 (Tyr172) polyclonal antibody (Product # PA5-143788). The antibody was detected using goat anti-rabbit DyLight® 594.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143788
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Vav2 (Tyr-172) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-172 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.125 mg/mL
Amount
12.5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-172) peptide before affinity purification using phospho-Vav2 (Tyr-172) peptide (without carrier). The antibody detects a 100 kDa* band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.
Target Information
VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Oncogene VAV2; unnamed protein product; vav 2 guanine nucleotide exchange factor; vav 2 oncogene; VAV oncogene homolog; Vav2; Vav2 oncogene
Gene Aliases: 2810040F13Rik; VAV-2
UniProt ID: (Human) P52735, (Mouse) Q60992
Entrez Gene ID: (Human) 7410, (Rat) 296603, (Mouse) 22325
immune response-regulating cell surface receptor signaling pathway
signal transduction
small GTPase mediated signal transduction
cell migration
lamellipodium assembly
intracellular signal transduction
positive regulation of protein kinase B signaling
response to xenobiotic stimulus
platelet activation
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
vascular endothelial growth factor receptor signaling pathway
regulation of small GTPase mediated signal transduction
cellular response to xenobiotic stimulus
regulation of intracellular signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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