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Invitrogen
BRO1 Polyclonal Antibody
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FIGURE: 1 / 1
BRO1 Antibody (PA5-144449) in WB
Western blot analysis of BRO1 in recombinant protein using a dilution of 1:2,000. Samples were incubated with BRO1 polyclonal antibody (Product # PA5-144449). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 49.9 kDa. Observed band size: 54 kDa.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-144449
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Arabidopsis thaliana BRO1 protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
5 mg/mL
Amount
500 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen (Probable). Associates with FREE1 and ELC to perform function in the ESCRT-I complex. Binds ubiquitin in vitro (PubMed:26902184). Plays a role in the biogenesis of vacuole and multivesicular bodies (MVBs) (PubMed:26342016, PubMed:26324913). Required for the endosomal location of AMSH3 (PubMed:26324913). Mediates high-affinity phosphate transporter trafficking to maintain phosphate homeostasis. Regulates vacuolar degradation of PHT1-1 (PubMed:26342016).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALIX; SPHR1
Gene Aliases: AT1G15130; F9L1.7; F9L1_7
UniProt ID: (Arabidopsis) F4HXZ1
Entrez Gene ID: (Arabidopsis) 838077
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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