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Invitrogen
MBD5 Polyclonal Antibody
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FIGURE: 1 / 3
MBD5 Antibody (PA5-148213) in ICC/IF
Immunocytochemistry analysis of MBD5 in Hela cells. Samples were incubated with MBD5 polyclonal antibody (Product # PA5-148213) using a dilution of 1:1,000 at 4°C overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-148213
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human MBD5
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
100 µg/mL
Amount
10 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
MBD5 binds to heterochromatin but does not interact with either methylated or unmethylated DNA (in vitro).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ11113; FLJ30517; methyl-CpG-binding protein MBD5; unnamed protein product
Gene Aliases: C2DELq23.1; DEL2Q23.1; MRD1
UniProt ID: (Human) Q9P267
Entrez Gene ID: (Human) 55777
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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