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Invitrogen
PIWIL1 Polyclonal Antibody
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FIGURE: 1 / 8
PIWIL1 Antibody (PA5-17034) in ICC/IF
Immunofluorescence analysis of Piwi-like protein 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PIWIL1 Polyclonal Antibody (Product # PA5-17034) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17034
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
85 µg/mL
Amount
8.5 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Hiwi is a member of the Piwi /Argonaute protein family. Hiwi is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis. Hiwi is also expressed in CD34+ hematopoietic progenitors, but not in CD34- hematopoietic cells, suggesting a role for this gene in the maintenance of hematopoietic stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CT80; HILI; member of the PIWI family; mili; Miwi like; Miwi likw; piwi homolog; piwi like homolog 1; piwi-like 1; piwi-like homolog 1; Piwi-like protein; piwi-like protein 2; PIWIL1L; piwil2-like protein; unnamed protein product
Gene Aliases: CT80.1; HIWI; MIWI; PIWI
UniProt ID: (Human) Q96J94, (Mouse) Q9JMB7
Entrez Gene ID: (Human) 9271, (Rat) 363912, (Mouse) 57749
spermatogenesis
spermatid development
gene silencing by RNA
piRNA metabolic process
sperm chromatin condensation
primary piRNA processing
piRNA-mediated gene silencing by mRNA destabilization
transposable element silencing by piRNA-mediated heterochromatin formation
piRNA-mediated heterochromatin formation
transposable element silencing by heterochromatin formation
regulation of cytoplasmic translation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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