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Invitrogen
ACSL1 Polyclonal Antibody
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FIGURE: 1 / 8
ACSL1 Antibody (PA5-17136) in ICC/IF
Immunofluorescence analysis of ACSL1 was performed using 70% confluent log phase MCF-10A and MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ACSL1 Polyclonal Antibody (Product # PA5-17136) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Prod... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17136
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a sequence of human ACSL1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
36 µg/mL
Amount
3.6 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetate-CoA ligase; acetyl-CoA synthetase; acetyl-Coenzyme A synthetase 1 (ADP forming); ACSL1 isoform a; ACSL1 isoform c; Acyl CoA synthetase, long chain; acyl-CoA synthetase 1; arachidonate--CoA ligase; fatty acid Coenzyme A ligase, long chain 2; fatty-acid-Coenzyme A ligase, long-chain 1; fatty-acid-Coenzyme A ligase, long-chain 2; L LACS2; LACS 1; LACS 2; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 1; long-chain acyl-CoA synthetase 2; long-chain fatty acid-CoA ligase 2; long-chain fatty-acid-coenzyme A ligase 1; long-chain-fatty-acid--CoA ligase, liver isozyme; OTTHUMP00000218457; OTTHUMP00000218460; OTTHUMP00000218462; OTTHUMP00000218465; palmitoyl-CoA ligase 1; palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1; phytanate--CoA ligase; unnamed protein product
Gene Aliases: Acas; Acas1; ACS; ACS1; COAA; FACL1; FACL2; FACS; LACS; LACS 1; LACS1; LACS2
UniProt ID: (Human) P33121, (Rat) P18163, (Mouse) P41216
Entrez Gene ID: (Human) 2180, (Rat) 25288, (Mouse) 14081
very long-chain fatty acid metabolic process
long-chain fatty acid metabolic process
fatty acid metabolic process
response to nutrient
lipid biosynthetic process
response to xenobiotic stimulus
positive regulation of plasma membrane long-chain fatty acid transport
fatty acid transport
triglyceride biosynthetic process
response to oleic acid
long-chain fatty-acyl-CoA biosynthetic process
xenobiotic catabolic process
long-chain fatty acid import
positive regulation of cold-induced thermogenesis
fatty acid biosynthetic process
adiponectin-activated signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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