Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Who We Serve
    • Biotech
    • Biopharma
    • CDMO
    • Lab Diagnostics
    • Industrial and Applied Sciences
  • Promotions
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • Order Status
          • Quick Order
          • Promos
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central
          • Primary Antibodies ›
          • p53 Antibodies

          Invitrogen

          Acetyl-p53 (Lys379) Polyclonal Antibody

          Advanced Verification
          2 Published Figures
          3 References
          View all (370) p53 antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Datasheet
          Certificates
          Protocols
          Questions & Answers

          Cite Acetyl-p53 (Lys379) Polyclonal Antibody

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (3)
          • Published Figures (2)
          • Advanced Verification (2)
          Acetyl-p53 (Lys379) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Acetyl-p53 (Lys379) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Acetyl-p53 (Lys379) Antibody (PA5-17287) in ICC/IF

          Immunofluorescence analysis of Acetyl-p53 (Lys379) was performed using 70% confluent log phase HeLa cells treated with 0.5uM Doxorubicin and 400nM Trichostatin A for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-p53 (Lys379) Polyclonal Ant... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Acetyl-p53 (Lys379) Antibody in Immunocytochemistry (ICC/IF)
          Acetyl-p53 (Lys379) Antibody in Western Blot (WB)
          Acetyl-p53 (Lys379) Antibody in ChIP Assay (ChIP)
          Acetyl-p53 (Lys379) Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))
          Acetyl-p53 (Lys379) Antibody in Western Blot (WB)
          Acetyl-p53 (Lys379) Antibody
          Acetyl-p53 (Lys379) Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Acetyl-p53 (Lys379) Polyclonal Antibody

          Product Details

          PA5-17287

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          -

          ChIP assay (ChIP)

          2 µL/10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic acetylated peptide corresponding to residues surrounding Lys379 of mouse p53
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          163 µg/mL

          Amount

          16.3 µg

          Purification

          Antigen affinity chromatography, Protein A

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10983350

          Product Specific Information

          It is not recommended to aliquot this antibody.

          Target Information

          The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: antigen NY-CO-13; Cys 51 Stop; FLJ92943; HGNC11998; ME-delta123p53; MLLP53; mutant; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p53; p53 antigen; p53 cellular tumor antigen; phosphoprotein p53; transcription factor; transcription factor; truncated protein; transformation-related protein 53; tumor protein 53; tumor suppressor; tumor suppressor p53; tumor supressor p53; Tumour Protein p53; unnamed protein product

          View more View less

          Gene Aliases: bbl; BCC7; bfy; bhy; BMFS5; LFS1; p44; P53; Tp53; TRP53

          View more View less

          UniProt ID: (Human) P04637, (Mouse) P02340

          View more View less

          Entrez Gene ID: (Human) 7157, (Mouse) 22059

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding TFIID-class transcription factor binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding mRNA 3'-UTR binding copper ion binding protein binding zinc ion binding enzyme binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone deacetylase regulator activity annealing helicase activity identical protein binding histone deacetylase binding protein heterodimerization activity chaperone binding protein phosphatase 2A binding RNA polymerase II sequence-specific DNA binding transcription factor binding 14-3-3 protein binding MDM2/MDM4 family protein binding disordered domain specific binding general transcription initiation factor binding molecular function activator activity molecular condensate scaffold activity promoter-specific chromatin binding transcription cofactor binding sequence-specific DNA binding ion channel binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter in utero embryonic development somitogenesis release of cytochrome c from mitochondria hematopoietic progenitor cell differentiation T cell proliferation involved in immune response B cell lineage commitment T cell lineage commitment positive regulation of leukocyte migration response to ischemia DNA repair nucleotide-excision repair double-strand break repair regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein import into nucleus autophagy apoptotic process cellular response to DNA damage stimulus response to oxidative stress ER overload response transforming growth factor beta receptor signaling pathway Ras protein signal transduction spermatogenesis regulation of mitotic cell cycle gastrulation negative regulation of neuroblast proliferation central nervous system development heart development circadian rhythm protein localization negative regulation of DNA replication negative regulation of cell proliferation determination of adult lifespan mRNA transcription rRNA transcription response to xenobiotic stimulus response to UV response to salt stress embryo development ending in birth or egg hatching response to X-ray response to gamma radiation gene expression positive regulation of gene expression negative regulation of gene expression positive regulation of cardiac muscle cell apoptotic process viral process cerebellum development negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator negative regulation of transforming growth factor beta receptor signaling pathway mitotic G1 DNA damage checkpoint negative regulation of telomere maintenance via telomerase T cell differentiation in thymus tumor necrosis factor-mediated signaling pathway cellular response to stress regulation of tissue remodeling cellular response to reactive oxygen species cellular response to UV multicellular organism growth positive regulation of mitochondrial membrane permeability regulation of cell proliferation cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod mitochondrial DNA repair regulation of DNA damage response, signal transduction by p53 class mediator regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of cell cycle positive regulation of gene expression, epigenetic negative regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter response to antibiotic fibroblast proliferation negative regulation of fibroblast proliferation cell development circadian behavior bone marrow development embryonic organ development negative regulation of smooth muscle cell proliferation protein stabilization negative regulation of DNA metabolic process protein tetramerization chromosome organization neuron apoptotic process regulation of intracellular pH regulation of cell cycle hematopoietic stem cell differentiation interferon-gamma-mediated signaling pathway cardiac septum morphogenesis positive regulation of programmed necrotic cell death protein-containing complex assembly intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of thymocyte apoptotic process positive regulation of thymocyte apoptotic process necroptotic process cellular response to hypoxia cellular response to xenobiotic stimulus cellular response to ionizing radiation cellular response to gamma radiation cellular response to UV-C signal transduction by p53 class mediator intrinsic apoptotic signaling pathway by p53 class mediator cellular response to actinomycin D positive regulation of release of cytochrome c from mitochondria cellular senescence replicative senescence oxidative stress-induced premature senescence apoptotic signaling pathway oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis negative regulation of macromitophagy regulation of mitochondrial membrane permeability involved in apoptotic process regulation of intrinsic apoptotic signaling pathway by p53 class mediator regulation of cell cycle G2/M phase transition positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of G1 to G0 transition negative regulation of production of miRNAs involved in gene silencing by miRNA negative regulation of glucose catabolic process to lactate via pyruvate negative regulation of pentose-phosphate shunt intrinsic apoptotic signaling pathway in response to hypoxia regulation of fibroblast apoptotic process negative regulation of DNA biosynthetic process negative regulation of reactive oxygen species metabolic process positive regulation of reactive oxygen species metabolic process regulation of cellular senescence positive regulation of cellular senescence regulation of intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway response to ionizing radiation negative regulation of helicase activity intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Social Responsibility Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy Consumer Health Data Privacy Policy
          • Policies and Notices
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Notice
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-blue-76c4fffd86-nv6df:80/100.66.78.131:80.
          git-commit: bdc067a1e9e92d056899a2dda64af631988aa8a1
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.54.0-Offline