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          • Primary Antibodies ›
          • RAC1/RAC2/RAC3 Antibodies

          Invitrogen

          RAC1/RAC2/RAC3 Polyclonal Antibody

          1 Published Figure
          2 References
          View all (8) RAC1/RAC2/RAC3 antibodies

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          Cite RAC1/RAC2/RAC3 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (1)
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          RAC1/RAC2/RAC3 Antibody (PA5-17519) in WB

          Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), U-87 MG (Lane 4), 3T3-L1 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-RAC1/RAC2/RAC2 Polyclonal Antibody (Product # PA5-17519, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 20 kDa band corresponding to RAC1/RAC2/RAC3 was observed across the cell lin... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          RAC1/RAC2/RAC3 Polyclonal Antibody

          Product Details

          PA5-17519

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Non-human primate, Rat, Xenopus

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues surrounding Thr138 of human Rac1
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          39 µg/mL

          Amount

          3.9 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10986181

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with other small GTPases.

          Target Information

          The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cell migration-inducing gene 5 protein; EN-7 protein; p21-Rac1; p21-Rac2; p21-Rac3; protein EN-7; Rac1ins; RAC2; ras-like protein; ras-like protein TC25; RAS-related C3 botulinum substrate 1; RAS-related C3 botulinum substrate 2; RAS-related C3 botulinum substrate 3; ras-related C3 botulinum toxin substrate; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) L homeolog; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3); Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac1; rho family, small GTP binding protein Rac3; small G protein; small GTP binding protein; Rac1; Rho-related protein; unnamed protein product

          View more View less

          Gene Aliases: D5Ertd559e; EN-7; Gx; HSPC022; IMD73A; IMD73B; IMD73C; MIG5; MRD48; p21-Rac1; p21-Rac2; rac; Rac-1; rac1; Rac1B; TC-25; XELAEV_18045321mg; Xrac

          View more View less

          UniProt ID: (Bovine) P62998, (Human) P63000, (Human) P15153, (Bovine) Q9TU25, (Human) P60763, (Mouse) P63001, (Rat) Q6RUV5, (Mouse) Q05144, (Mouse) P60764

          View more View less

          Entrez Gene ID: (Bovine) 281440, (Human) 5879, (Human) 5880, (Bovine) 327671, (Human) 5881, (Bovine) 619066, (Mouse) 19353, (Rat) 363875, (Rat) 366957, (Mouse) 19354, (Mouse) 170758, (Rat) 688319, (Xenopus) 734382

          View more View less

          Function(s)
          protein binding GTP binding Rho GDP-dissociation inhibitor binding GTPase activity obsolete small monomeric GTPase activity enzyme binding protein kinase binding GTP-dependent protein binding thioesterase binding macromolecular complex binding protein kinase regulator activity calcium-dependent protein binding small GTPase binding histone deacetylase binding ATPase binding small GTPase
          Process(es)
          neuron migration positive regulation of protein phosphorylation regulation of cell migration cell motility semaphorin-plexin signaling pathway sphingosine-1-phosphate signaling pathway phagocytosis, engulfment inflammatory response actin filament organization cell adhesion cell-matrix adhesion establishment or maintenance of cell polarity enzyme linked receptor protein signaling pathway small GTPase mediated signal transduction motor neuron axon guidance regulation of cell shape response to wounding anatomical structure morphogenesis regulation of hydrogen peroxide metabolic process regulation of lamellipodium assembly positive regulation of lamellipodium assembly positive regulation of endothelial cell migration regulation of fibroblast migration negative regulation of fibroblast migration regulation of cell-substrate adhesion cell migration Rac protein signal transduction cell projection assembly lamellipodium assembly actin cytoskeleton organization actin filament polymerization cortical cytoskeleton organization forebrain development ruffle organization response to lipopolysaccharide negative regulation of interleukin-23 production regulation of actin cytoskeleton organization substrate adhesion-dependent cell spreading positive regulation of Rho protein signal transduction intracellular signal transduction non-canonical Wnt signaling pathway positive regulation of insulin secretion involved in cellular response to glucose stimulus superoxide anion generation engulfment of apoptotic cell regulation of nitric oxide biosynthetic process respiratory burst regulation of JAK-STAT cascade hepatocyte growth factor receptor signaling pathway negative regulation of receptor-mediated endocytosis neuron projection morphogenesis regulation of stress fiber assembly positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly Wnt signaling pathway, planar cell polarity pathway regulation of respiratory burst cell chemotaxis regulation of cell adhesion involved in heart morphogenesis positive regulation of neutrophil chemotaxis ruffle assembly regulation of postsynapse assembly positive regulation of substrate adhesion-dependent cell spreading positive regulation of ruffle assembly regulation of synaptic vesicle endocytosis regulation of neutrophil migration positive regulation of bicellular tight junction assembly midbrain dopaminergic neuron differentiation positive regulation of ovarian follicle development chemotaxis signal transduction regulation of T cell proliferation regulation of mast cell degranulation bone resorption regulation of mast cell chemotaxis lymphocyte aggregation positive regulation of establishment of protein localization to mitochondrion G-protein coupled receptor signaling pathway positive regulation of cell proliferation regulation of protein kinase activity Wnt signaling pathway positive regulation of cell adhesion mediated by integrin postsynaptic actin cytoskeleton organization auditory receptor cell morphogenesis mast cell chemotaxis epithelial cell morphogenesis endocytosis hyperosmotic response cytoskeleton organization axon guidance positive regulation of cell-substrate adhesion regulation of neuron maturation dendrite development cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development regulation of cell morphogenesis positive regulation of actin filament polymerization positive regulation of microtubule polymerization angiotensin-activated signaling pathway erythrocyte enucleation cell-cell junction organization positive regulation of DNA replication regulation of neuronal synaptic plasticity anatomical structure arrangement dendrite morphogenesis homeostasis of number of cells homeostasis of number of cells within a tissue positive regulation of filopodium assembly positive regulation of protein kinase B signaling synaptic transmission, GABAergic positive regulation of dendritic spine development regulation of ERK5 cascade cellular response to mechanical stimulus dopaminergic neuron differentiation protein localization to plasma membrane cochlea morphogenesis multicellular organismal homeostasis neuron projection development neuromuscular process controlling balance neuromuscular process
          It has to be done as per old AB suggested Products section.

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