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Invitrogen

Phospho-IKK alpha/beta (Ser176, Ser180) Polyclonal Antibody

View all (17) IKK alpha/beta antibodies

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Product Details

PA5-17593

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phosphopeptide corresponding to a region surrounding pSer177/181 of IKKβ

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

13.8 µg/mL

Amount

1.38 µg

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978564

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKK-a kinase; IKK-B; IKK-beta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; NFKBIKB; nuclear factor kappa B kinase subunit beta; nuclear factor NF-kappa-B inhibitor kinase alpha; nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; protein kinase; serine/threonine protein kinase IKBKB; TCF17; transcription factor 16; unnamed protein product

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Gene Aliases: AIM-1; BPS2; Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha; IKK-1; IKK-2; IKK-alpha; IKK-B; IKK-beta; IKK1; IKK2; IKK[b]; IKKA; IKKB; IKKbeta; IMD15; IMD15A; IMD15B; NFKBIKA; NFKBIKB; TCF16

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UniProt ID: (Human) O15111, (Human) O14920, (Mouse) O88351, (Rat) Q9QY78

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Entrez Gene ID: (Human) 1147, (Mouse) 12675, (Rat) 309361, (Human) 3551, (Mouse) 16150, (Rat) 84351

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding IkappaB kinase activity protein homodimerization activity macromolecular complex binding protein heterodimerization activity scaffold protein binding transferrin receptor binding identical protein binding nucleotide binding protein kinase binding protein serine kinase activity protein phosphatase binding
Process(es)
morphogenesis of an epithelial sheet regulation of transcription from RNA polymerase II promoter integrin-mediated signaling pathway I-kappaB kinase/NF-kappaB signaling I-kappaB phosphorylation lactation osteoclast differentiation positive regulation of interferon-alpha production tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation toll-like receptor 4 signaling pathway response to muscle stretch NIK/NF-kappaB signaling odontogenesis of dentin-containing tooth positive regulation of I-kappaB kinase/NF-kappaB signaling skin development positive regulation of transcription from RNA polymerase II promoter striated muscle cell differentiation defense response to virus mammary gland alveolus development response to cholecystokinin cellular response to tumor necrosis factor cellular response to virus protein polyubiquitination B cell homeostasis protein phosphorylation inflammatory response positive regulation of cell proliferation positive regulation of sodium ion transport positive regulation of neuron projection development peptidyl-serine phosphorylation signal transduction involved in regulation of gene expression neuron projection development negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of transcription, DNA-templated protein maturation interleukin-1-mediated signaling pathway protein localization to plasma membrane negative regulation of cytokine production involved in inflammatory response regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly pattern recognition receptor signaling pathway skeletal muscle contraction immune response response to xenobiotic stimulus response to virus response to toxic substance anatomical structure morphogenesis response to acetate response to hydroperoxide response to amino acid innate immune response stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent MyD88-dependent toll-like receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of toll-like receptor signaling pathway toll-like receptor 3 signaling pathway TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway T cell receptor signaling pathway stress-activated MAPK cascade response to lipopolysaccharide response to fatty acid
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