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Invitrogen

Phospho-NPM1 (Thr199) Polyclonal Antibody

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Phospho-NPM1 (Thr199) Polyclonal Antibody

Product Details

PA5-17736

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues around pThr199 of human NPM
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

44 µg/mL

Amount

4.4 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980331

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B23 antibody; deletion in NPMc- AML; MGC104254; mNPM antibody; mNPM1 antibody; mutation A in NPMc+ AML; mutation B in NPMc+ AML; mutation C in NPMc+ AML; mutation D in NPMc+ AML; mutation E in NPMc+ AML; mutation F in NPMc+ AML; NO38 antibody; NPM; nucleolar phosphoprotein B23; nucleolar protein B23; nucleolar protein B23.1; nucleolar protein B23.2; nucleolar protein NO38; nucleophosmin (nucleolar phosphoprotein B23 numatrin) isoform NPM1_1; nucleophosmin (nucleolar phosphoprotein B23 numatrin) isoform NPM1_2; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; numatrin; numatrin antibody; testicular tissue protein Li 128; unnamed protein product

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Gene Aliases: B23; B23NP; NO38; NPM

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UniProt ID: (Human) P06748, (Mouse) Q61937, (Rat) P13084

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Entrez Gene ID: (Human) 4869, (Mouse) 18148, (Rat) 25498

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Function(s)
core promoter sequence-specific DNA binding nucleic acid binding chromatin binding transcription coactivator activity RNA binding protein kinase inhibitor activity protein binding rRNA binding protein kinase binding Tat protein binding histone binding identical protein binding protein homodimerization activity ribosomal large subunit binding ribosomal small subunit binding NF-kappaB binding binding, bridging DNA-binding transcription factor binding p53 binding DNA binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding enzyme binding protein kinase B binding
Process(es)
ribosomal large subunit export from nucleus ribosomal small subunit export from nucleus cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) regulation of cell growth DNA repair nucleosome assembly chromatin remodeling protein import into nucleus cell volume homeostasis nucleocytoplasmic transport centrosome cycle I-kappaB kinase/NF-kappaB signaling protein localization positive regulation of cell proliferation negative regulation of cell proliferation rRNA transcription posttranscriptional regulation of gene expression negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process regulation of centrosome duplication positive regulation of centrosome duplication negative regulation of centrosome duplication macrophage differentiation positive regulation of protein ubiquitination regulation of protein stability cellular response to UV regulation of cell proliferation ribosomal large subunit biogenesis ribosomal small subunit biogenesis negative regulation of apoptotic process positive regulation of catalytic activity regulation of DNA damage response, signal transduction by p53 class mediator regulation of neuron apoptotic process negative regulation of neuron apoptotic process negative regulation of protein kinase activity by regulation of protein phosphorylation positive regulation of translation positive regulation of DNA replication positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of centriole replication negative regulation of mRNA splicing, via spliceosome negative regulation of epithelial cell proliferation protein stabilization positive regulation of DNA metabolic process regulation of cell cycle regulation of eIF2 alpha phosphorylation by dsRNA cellular senescence positive regulation of DNA-directed DNA polymerase activity regulation of mRNA stability involved in cellular response to UV positive regulation of cell cycle G2/M phase transition positive regulation of protein localization to nucleolus positive regulation of cytoplasmic translation intracellular protein transport signal transduction ribosome biogenesis ribosome assembly cardiac muscle hypertrophy cellular response to testosterone stimulus cellular response to hypoxia liver regeneration
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