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N-cadherin Polyclonal Antibody
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FIGURE: 1 / 12
N-cadherin Antibody (PA5-19486) in ICC/IF
Immunofluorescence analysis of N-cadherin was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Monoclonal Antibody (Product # PA5-19486) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius ov... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-19486
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and cow based on sequence homology.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CDw325 antigen; N-cadherin; N-cadherin 1; neural cadherin; neural cadherin precursor; unnamed protein product
Gene Aliases: ACOGS; ADHD8; ARVD14; CD325; CDHN; CDw325; N-CAD; N-cadherin; NCAD
UniProt ID: (Human) P19022, (Rat) Q9Z1Y3, (Mouse) P15116
Entrez Gene ID: (Human) 1000, (Rat) 83501, (Mouse) 12558
RNA binding
calcium ion binding
protein binding
beta-catenin binding
enzyme binding
protein kinase binding
protein phosphatase binding
identical protein binding
alpha-catenin binding
gamma-catenin binding
cadherin binding
macromolecular complex binding
nitric-oxide synthase binding
protein tyrosine kinase binding
cell morphogenesis
type B pancreatic cell development
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
synapse assembly
glial cell differentiation
neural crest cell development
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
telencephalon development
cerebral cortex development
regulation of myelination
regulation of protein localization
adherens junction organization
regulation of Rho protein signal transduction
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
positive regulation of axon extension
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
modulation of synaptic transmission
striated muscle cell differentiation
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
negative regulation of canonical Wnt signaling pathway
mesenchymal cell migration
synaptic vesicle clustering
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
cell-cell adhesion
regulation of postsynaptic density protein 95 clustering
positive regulation of cellular protein localization
positive regulation of synaptic vesicle clustering
detection of muscle stretch
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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