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Invitrogen
IRF5 Polyclonal Antibody
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FIGURE: 1 / 3
IRF5 Antibody (PA5-19504) in ICC/IF
Immunofluorescence analysis of IRF5 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IRF5 Polyclonal Antibody (Product # PA5-19504) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-19504
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to BSA derived from within residues 450 to the C-terminus of Human IRF5.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.9 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Not suitable for use with Immunohistochemistry (Paraffin).
This antibody is predicted to react with cow based on sequence homology.
Target Information
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: interferon regulatory factor 5 bone marrow; OTTHUMP00000212907; transcription factor; unnamed protein product
Gene Aliases: mirf5; SLEB10
UniProt ID: (Human) Q13568, (Mouse) P56477
Entrez Gene ID: (Human) 3663, (Mouse) 27056, (Rat) 296953
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
identical protein binding
sequence-specific DNA binding
protein serine/threonine kinase binding
sequence-specific double-stranded DNA binding
immune system process
positive regulation of cytokine production involved in immune response
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cytokine-mediated signaling pathway
positive regulation of type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
toll-like receptor 9 signaling pathway
positive regulation of apoptotic process
innate immune response
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to peptidoglycan
cellular response to muramyl dipeptide
cellular response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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