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Invitrogen
RIP2 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-19933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to amino acids 508 to 522 of human origin.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot applications, this antibody detects a band at ~60kDa. A suggested positive control is K562 cell lysate.
PA5-19933 can be used with blocking peptide PEP-0058.
Target Information
RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD domain protein; phosphorylated protein; CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARDIAK; RIP-like kinase; EC 2.7.11.1; growth-in; growth-inhibiting gene 30; kinase RIPK2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; receptor-interacting protein 2; RIP 2; RIP-2; RIP-like; RIP2; serine/threonine kinase homolog; contains CARD motif; tyrosine-protein kinase RIPK2; unnamed protein product; WUGSC:H_RG437L15.1
Gene Aliases: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2
UniProt ID: (Human) O43353
Entrez Gene ID: (Human) 8767
protein kinase activity
protein serine/threonine kinase activity
JUN kinase kinase kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
LIM domain binding
signaling adaptor activity
identical protein binding
protein homodimerization activity
CARD domain binding
caspase binding
protein serine kinase activity
MAPK cascade
positive regulation of cytokine production
adaptive immune response
apoptotic process
inflammatory response
signal transduction
I-kappaB kinase/NF-kappaB signaling
positive regulation of protein ubiquitination
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
toll-like receptor 2 signaling pathway
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
protein homooligomerization
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to muramyl dipeptide
cell surface toll-like receptor signaling pathway
positive regulation of protein K63-linked ubiquitination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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