Invitrogen

SCXA Polyclonal Antibody

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5 Published Figures

8 References

View all (7) SCXA antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Additional Information:

SCXA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SCXA Antibody (PA5-23943) in IHC (P)

Immunohistochemistry analysis of SCXA in paraffin-embedded Human breast carcinoma tissue. Samples were incubated with SCXA polyclonal antibody (Product # PA5-23943) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0)... View More

SCXA Antibody in Immunocytochemistry (ICC/IF)

SCXA Antibody in Western Blot, ChIP Assay (WB, ChIP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SCXA Antibody — Figure 3 cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. ( a ) Immunoblot analysis of indicated proteins from fibroblasts treated with cANGPTL4 (12 ug/mL) in the presence or absence of TGFbeta1 (10 ng/mL). beta-tubulin, as loading control, was from the same samples. ( b,d ) Chromatin immunoprecipitation (ChIP) of SBE sites in the human COL1A2 gene promoter region and E-box sites in the human COL1A2 and COL3A1 gene promoter regions using phospho-Smad3 antibodies and scleraxis (ScxA) antibodies respectively. The gene sequences spanning the SBE and E-box sites and a random control sequence were analyzed by PCR from immunoprecipitated chromatin from fibroblasts treated with TGFbeta1 in the presence or absence of cANGPTL4 (cANG). Pre-immune serum was used as a control. PCR was performed using chromatin before immunoprecipitation as input. M, 100 bp DNA ladder. ( c ) A schematic diagram showing the relative positions of Enhancer boxes (E-Boxes) and Smad binding elements (SBE) on the human COL1A2 and COL3A1 proximal promoters. Sequences of E-Boxes and SBE are shown. ChIP PCR primers designed specifically to bind scleraxis along this proximal promoter region are indicated. ( e ) Relative mRNA expression of ID3 in fibroblasts treated with 12 ug/mL of cANGPTL4 and lysed for mRNA at the indicated time points post-treatment. ( f ) Relative mRNA expression of ID3 in control (F ctrl ) and ID3-knockdown (F [delta]ID3 ) fibroblasts treated {TM}

Immunogen image

Immunogen Image SCXA Polyclonal Antibody

Product Details

PA5-23943

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human SCXA

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

360 µg

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2541443

Product Specific Information

This antibody is predicted to react with chicken based on sequence homology.

Target Information

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: basic helix-loop-helix transcription factor; class A basic helix-loop-helix protein 41; class A basic helix-loop-helix protein 48; class II bHLH protein scleraxis; scleraxis; scleraxis basic helix-loop-helix transcription factor; scleraxis homolog A; scleraxis homolog B

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Gene Aliases: Bhlha41; bHLHa48; Scl; SCXA; SCXB

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UniProt ID: (Human) Q7RTU7, (Mouse) Q64124

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Entrez Gene ID: (Human) 642658, (Mouse) 20289

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding bHLH transcription factor binding sequence-specific DNA binding protein dimerization activity E-box binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding

Process(es)

mesoderm formation tissue homeostasis endochondral ossification chondrocyte differentiation heart valve morphogenesis heart valve formation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression cell differentiation collagen fibril organization BMP signaling pathway developmental process positive regulation of collagen biosynthetic process tendon development tendon cell differentiation tendon formation deltoid tuberosity development negative regulation of apoptotic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter Sertoli cell differentiation Sertoli cell development regulation of cartilage development positive regulation of cartilage development cellular response to cAMP cellular response to follicle-stimulating hormone stimulus cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus positive regulation of gastrulation tissue development skeletal muscle cell differentiation negative regulation of transcription, DNA-templated embryonic skeletal system development cartilage development face morphogenesis sclerotome development muscle structure development cellular response to mechanical stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SCXA Polyclonal Antibody

SCXA Antibody (PA5-23943) in IHC (P)

Product Details

PA5-23943

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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