Search

  • Order Status
  • Quick Order
  • Promos

Invitrogen

PARP1 Polyclonal Antibody

Advanced Verification
View all (80) PARP1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Datasheet
Certificates
Protocols
Questions & Answers
Datasheet
Certificates
Protocols
Questions & Answers
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

PA5-27219

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

Assay-dependent
-

ChIP assay (ChIP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Amount

30 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544695

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, HeLa (30 µM cisplatin treatment for 24 hr), HCT116, HCT116 (30uM cisplatin treatment for 24hr), PC-12, Rat2.

Predicted reactivity: Mouse (86%), Rat (85%), Chicken (80%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; protein poly-ADP-ribosyltransferase PARP1; unnamed protein product

View more View less

Gene Aliases: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; ARTD1; msPARP; pADPRT-1; PARP; PARP-1; PARS; Poly-PARP; PPOL; sPARP-1

View more View less

UniProt ID: (Human) P09874, (Rat) P27008

View more View less

Entrez Gene ID: (Human) 142, (Mouse) 11545, (Rat) 25591

View more View less

Function(s)
DNA binding chromatin binding damaged DNA binding RNA binding NAD+ ADP-ribosyltransferase activity protein binding enzyme activator activity zinc ion binding enzyme binding protein kinase binding estrogen receptor binding nucleosome binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity histone deacetylase binding non-sequence-specific DNA binding, bending NAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding R-SMAD binding NAD DNA ADP-ribosyltransferase activity transcription regulator activator activity NAD+-protein-serine ADP-ribosyltransferase activity NAD+-protein-aspartate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity NAD+-protein-tyrosine ADP-ribosyltransferase activity NAD+-protein-histidine ADP-ribosyltransferase activity NAD+-histone H2BS6 serine ADP-ribosyltransferase activity NAD+-histone H3S10 serine ADP-ribosyltransferase activity NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity single-strand break-containing DNA binding protein ADP-ribosylase activity transferase activity, transferring pentosyl groups
Process(es)
single strand break repair negative regulation of transcription from RNA polymerase II promoter telomere maintenance DNA repair double-strand break repair transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway response to gamma radiation positive regulation of cardiac muscle hypertrophy carbohydrate biosynthetic process protein autoprocessing signal transduction involved in regulation of gene expression macrophage differentiation DNA ADP-ribosylation mitochondrial DNA metabolic process positive regulation of DNA-templated transcription, elongation cellular response to insulin stimulus regulation of protein localization positive regulation of intracellular estrogen receptor signaling pathway negative regulation of transcription elongation from RNA polymerase II promoter cellular response to oxidative stress cellular response to UV protein modification process positive regulation of I-kappaB kinase/NF-kappaB signaling mitochondrial DNA repair regulation of circadian sleep/wake cycle, non-REM sleep response to ethanol negative regulation of innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization positive regulation of mitochondrial depolarization positive regulation of SMAD protein import into nucleus positive regulation of necroptotic process protein poly-ADP-ribosylation protein auto-ADP-ribosylation protein localization to chromatin establishment of protein localization to chromatin cellular response to zinc ion cellular response to transforming growth factor beta stimulus replication fork reversal negative regulation of cGAS/STING signaling pathway positive regulation of protein localization to nucleus cellular response to oxygen-containing compound regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway response to aldosterone negative regulation of adipose tissue development negative regulation of telomere maintenance via telomere lengthening cellular response to beta-amyloid positive regulation of myofibroblast differentiation regulation of base-excision repair positive regulation of double-strand break repair via homologous recombination cellular response to nerve growth factor stimulus ATP generation from poly-ADP-D-ribose negative regulation of ATP biosynthetic process double-strand break repair via homologous recombination DNA metabolic process base-excision repair negative regulation of interleukin-17 production regulation of growth rate behavioral response to cocaine cellular response to superoxide DNA repair-dependent chromatin remodeling transcription pausing by RNA polymerase II regulation of single strand break repair protein localization to site of double-strand break
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support