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Invitrogen
14-3-3 zeta Polyclonal Antibody
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FIGURE: 1 / 14
14-3-3 zeta Antibody (PA5-27317) in ICC/IF
Immunofluorescence analysis of YWHAZ was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with 14-3-3 zeta Polyclonal Antibody (Product # PA5-27317) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsiu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27317
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.43 mg/mL
Amount
43 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3.
Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (89%), Pig (99%), Chicken (99%), Sheep (99%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 14-3-3 delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 1433Z; 143Z; epididymis luminal protein 4; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; mitochondrial import stimulation factor S1 subunit; phospholipase A2; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; unnamed protein product
Gene Aliases: 1110013I11Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; HEL-S-3; HEL-S-93; HEL4; KCIP-1; POPCHAS; YWHAD
UniProt ID: (Human) P63104, (Mouse) P63101, (Rat) P63102
Entrez Gene ID: (Human) 7534, (Mouse) 22631, (Rat) 25578
RNA binding
protein binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
ubiquitin protein ligase binding
identical protein binding
ion channel binding
cadherin binding
phosphoserine binding
DNA-binding transcription factor binding
protein sequestering activity
macromolecular complex binding
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
respiratory system process
protein phosphorylation
signal transduction
synaptic target recognition
protein localization
lung development
regulation of protein stability
tube formation
cellular response to glucose starvation
negative regulation of apoptotic process
negative regulation of innate immune response
establishment of Golgi localization
regulation of ERK1 and ERK2 cascade
regulation of synapse maturation
Golgi reassembly
negative regulation of protein localization to nucleus
negative regulation of TORC1 signaling
histamine secretion by mast cell
protein targeting
lysosome organization
response to xenobiotic stimulus
cellular response to nutrient levels
TORC1 signaling
regulation of programmed cell death
establishment of protein localization
ERK1 and ERK2 cascade
protein localization to mitochondrion
antibacterial innate immune response
regulation of lysosome organization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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