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Invitrogen

ILK Polyclonal Antibody

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View all (35) ILK antibodies

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Product Details

PA5-27484

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Amount

80 µg

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544960

Product Specific Information

Recommended positive controls: HeLa, A431, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (81%), Xenopus laevis (84%), Chicken (86%), Sheep (97%), Rhesus Monkey (100%), Bovine (98%), Guinea pig (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; epididymis secretory protein Li 28; ILK; inactive integrin-linked kinase; integrin binding protein kinase; Integrin linked ILK; integrin-linked kinase; integrin-linked kinase-2; integrin-linked protein kinase; loc; lost-contact; main squeeze; msq; unnamed protein product; zilk

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Gene Aliases: ESTM24; HEL-S-28; ILK-1; ILK-2; P59; p59ILK; zgc:65842

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UniProt ID: (Human) Q13418, (Rat) Q99J82, (Mouse) O55222

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Entrez Gene ID: (Human) 3611, (Rat) 170922, (Mouse) 16202, (Zebrafish) 393543

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity receptor binding integrin binding protein binding ATP binding protein kinase binding protein binding, bridging SH3 domain binding protein domain specific binding macromolecular complex binding kinase activity transferase activity
Process(es)
cell morphogenesis outflow tract morphogenesis mitotic spindle organization cell-matrix adhesion integrin-mediated signaling pathway anatomical structure morphogenesis positive regulation of signal transduction cell differentiation positive regulation of BMP signaling pathway tumor necrosis factor-mediated signaling pathway substrate adhesion-dependent cell spreading positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of osteoblast differentiation positive regulation of transcription, DNA-templated actin filament bundle assembly actin filament network formation platelet aggregation caveola assembly protein localization to cell cortex positive regulation of canonical Wnt signaling pathway positive regulation of substrate adhesion-dependent cell spreading regulation of cell growth branching involved in ureteric bud morphogenesis positive regulation of cell-matrix adhesion protein phosphorylation negative regulation of protein kinase activity positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of cardiac muscle cell apoptotic process fibroblast migration Schwann cell development negative regulation of smooth muscle cell migration peptidyl-serine phosphorylation nerve development myelination in peripheral nervous system positive regulation of cell migration regulation of actin cytoskeleton organization negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade protein kinase B signaling negative regulation of neuron apoptotic process establishment or maintenance of epithelial cell apical/basal polarity positive regulation of myoblast differentiation positive regulation of axon extension negative regulation of smooth muscle cell proliferation neuron projection morphogenesis positive regulation of axonogenesis positive regulation of dendrite morphogenesis positive regulation of protein kinase B signaling fibril organization negative regulation of neural precursor cell proliferation positive regulation of cellular senescence blood vessel development endothelial cell development cardiac ventricle formation heart development regulation of heart contraction muscle attachment phosphorylation tendon development cardiac muscle cell development heart contraction
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