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Invitrogen
Nrf2 Polyclonal Antibody
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FIGURE: 1 / 62
Nrf2 Antibody (PA5-27882) in ICC/IF
Immunofluorescence analysis of Nuclear factor erythroid 2-related factor 2 was performed using 70% confluent log phase Hep G2 cells treated with TBHQ (100 micromolar for 4h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nrf2 Polyclonal Antibody (Product # PA5-27882) at 1:100 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H... View More
Product Details
PA5-27882
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Human,
Mouse,
Plant,
Reptile,
Rat,
Zebrafish
Published species
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.26 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: C8D30, 3xFlag-human NFE2L2-transfected 293T, MDA-MB-231 nuclear extract, MDA-MB-231 nuclear extract (30 µM tBHQ, 4hr), HepG2 (10 µM MG132 treatment for 15 hr).
Predicted reactivity: Rhesus Monkey (99%), Bovine (84%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E4TF1-60; E4TF1A; NF-E2-like basic leucine zipper transcriptional activator; NF-E2-related factor 2; NFE2-related factor 2; NFT2; NRF2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2 like 2; Nuclear factor, erythroid derived 2, like 2; unnamed protein product
Gene Aliases: AI194320; HEBP1; IMDDHH; NFE2L2; Nrf-2; NRF2
UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968
Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein domain specific binding
ubiquitin protein ligase binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
molecular condensate scaffold activity
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
protein heterodimerization activity
basic leucine zipper transcription factor
response to ischemia
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
inflammatory response
response to oxidative stress
response to xenobiotic stimulus
gene expression
proteasomal ubiquitin-independent protein catabolic process
positive regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
positive regulation of neuron projection development
protein ubiquitination
positive regulation of blood coagulation
endoplasmic reticulum unfolded protein response
cellular response to oxidative stress
response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
cellular response to glucose starvation
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of blood vessel endothelial cell migration
regulation of innate immune response
cell redox homeostasis
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of embryonic development
aflatoxin catabolic process
positive regulation of glucose import
cellular response to methionine
response to caloric restriction
cellular response to hydrogen peroxide
cellular response to copper ion
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to xenobiotic stimulus
cellular response to fluid shear stress
cellular response to laminar fluid shear stress
reactive oxygen species metabolic process
negative regulation of ferroptosis
integrated stress response signaling
negative regulation of cellular response to hypoxia
regulation of cellular response to oxidative stress
negative regulation of hematopoietic stem cell differentiation
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of glutathione biosynthetic process
positive regulation of ERAD pathway
cellular response to angiotensin
negative regulation of vascular associated smooth muscle cell migration
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
regulation of removal of superoxide radicals
negative regulation of endothelial cell apoptotic process
positive regulation of reactive oxygen species metabolic process
transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to drug
positive regulation of transcription from RNA polymerase II promoter in response to stress
positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress
negative regulation of cell death
negative regulation of hydrogen peroxide-induced cell death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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