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Invitrogen
Stella Polyclonal Antibody
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FIGURE: 1 / 6
Stella Antibody (PA5-34601) in ICC/IF
Immunofluorescence analysis of Stella was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Stella Polyclonal Antibody (Product # PA5-34601) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-34601
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Mouse Stella.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.9 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For Western Blot, this antibody has non-specific bands at 125 kDa and 25 kDa.
This antibody is predicted to react with rat based on sequence homology.
Target Information
DPPa3 (stella-related protein) is a stem cell marker protein which is restricted to pluripotent human embryonic stem cells, premeiotic germ lineage cells and testicular germ cell tumor cells. Otherwise known as 'developmental pluripotency-associated protein 3,' it is expressed in primordial germ cells, Oocytes, preimplantation embryos and pluripotent cells. This protein has a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cap1p; compaction-associated protein 1; primordial germ cell 7; primordial germ cell protein 7; stella-related protein; unnamed protein product
Gene Aliases: 2410075G02Rik; PCG7; PGC7; STELLA
UniProt ID: (Human) Q6W0C5, (Mouse) Q8QZY3
Entrez Gene ID: (Human) 359787, (Mouse) 73708
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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