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Invitrogen
FOXD1 Polyclonal Antibody
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FIGURE: 1 / 10
FOXD1 Antibody (PA5-35145) in ICC/IF
Immunocytochemistry analysis of FOXD1 in HepG2 cells. Samples were incubated with FOXD1 polyclonal antibody (Product # PA5-35145) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXD1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
800 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
This intronless gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: brain factor 2; FORKHEAD BOX PROTEIN D1 (FORKHEAD-RELATED PROTEIN FKHL8) (FORKHEAD-RELATED TRANSCRIPTION FACTOR 4) (FREAC-4); Forkhead, drosophila, homolog-like 8; forkhead-like 8; forkhead-related activator 4; forkhead-related protein FKHL8; forkhead-related transcription factor 4; FREAC-4; HFH-BF-2; HNF-3/forkhead homolog, brain factor 2; unnamed protein product
Gene Aliases: BF-2; FKHL8; FREAC-4; FREAC4; Hfh10; Hfhbf2
UniProt ID: (Human) Q16676, (Mouse) Q61345
Entrez Gene ID: (Human) 2297, (Mouse) 15229
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
DNA binding, bending
sequence-specific DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
anatomical structure morphogenesis
positive regulation of gene expression
cell differentiation
positive regulation of BMP signaling pathway
luteinizing hormone secretion
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
canonical Wnt signaling pathway
dichotomous subdivision of terminal units involved in ureteric bud branching
nephrogenic mesenchyme development
metanephric nephron development
metanephric capsule development
metanephric capsule specification
pattern specification involved in metanephros development
positive regulation of kidney development
branching involved in ureteric bud morphogenesis
kidney development
axon guidance
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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