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Invitrogen
Phospho-DARPP-32 (Ser137) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-DARPP-32 (Ser137) Antibody (PA5-35384) in WB
Western blot of DARPP-32 in rat striatal lysate showing specific immunolabeling of a band at ~32 kDa corresponding to Phospho-DARPP-32 (Ser137) polyclonal antibody (Product # PA5-35384) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by pre-adsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not show... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35384
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 of rat PPP1R1B conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.1mg/mL BSA, 0.15M NaCl, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, human, mouse and non-human primate based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dopamine and cAMP regulated phosphoprotein; dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; unnamed protein product
Gene Aliases: DARPP-32; Darpp32
UniProt ID: (Mouse) Q60829, (Rat) Q6J4I0
Entrez Gene ID: (Mouse) 19049, (Rat) 360616
response to amphetamine
transcription, DNA-templated
signal transduction
memory
negative regulation of female receptivity
locomotory behavior
visual learning
response to nicotine
intracellular signal transduction
response to cocaine
response to morphine
behavioral response to cocaine
cellular response to cocaine
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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