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Invitrogen
Phospho-HDAC2 (Ser394) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-HDAC2 (Ser394) Antibody (PA5-35395) in WB
Western blot of HDAC2 in mouse heart lysate showing specific immunolabeling of a band at ~55 kDa corresponding to Phospho-HDAC2 (Ser394) polyclonal antibody (Product # PA5-35395) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35395
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 of human HDAC2 conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
~0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/mL BSA
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, chicken, guinea pig, non-human primate, rat and sheep based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Target Information
HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HD2; hdac 2; hdac-2; protein deacylase HDAC2; transcriptional regulator homolog RPD3; unnamed protein product; YY1 transcription factor-binding protein; YY1-associated factor 1
Gene Aliases: D10Wsu179e; HD2; KDAC2; mRPD3; RPD3; YAF1; Yy1bp
UniProt ID: (Human) Q92769, (Mouse) P70288
Entrez Gene ID: (Human) 3066, (Mouse) 15182, (Rat) 84577
transcription cofactor binding
chromatin binding
RNA binding
histone deacetylase activity
protein binding
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
enzyme binding
nucleosomal DNA binding
protein deacetylase activity
histone binding
histone deacetylase binding
NF-kappaB binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
protein decrotonylase activity
histone decrotonylase activity
protein de-2-hydroxyisobutyrylase activity
protein lysine delactylase activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
deacetylase activity
heat shock protein binding
chromatin DNA binding
histone deacetylase activity (H4-K16 specific)
Krueppel-associated box domain binding
DNA-binding transcription factor binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
chromatin remodeling
positive regulation of cell proliferation
epidermal cell differentiation
positive regulation of epithelial to mesenchymal transition
negative regulation of neuron projection development
dendrite development
hippocampus development
negative regulation of Wnt signaling pathway
neuron differentiation
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of protein modification process
heterochromatin assembly
positive regulation of interleukin-1 production
positive regulation of tumor necrosis factor production
circadian regulation of gene expression
positive regulation of intracellular estrogen receptor signaling pathway
cellular response to trichostatin A
odontogenesis of dentin-containing tooth
regulation of cell fate specification
embryonic digit morphogenesis
negative regulation of apoptotic process
negative regulation of gene expression, epigenetic
positive regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of oligodendrocyte differentiation
progesterone receptor signaling pathway
regulation of protein kinase B signaling
cardiac muscle cell development
negative regulation of cardiac muscle cell proliferation
regulation of sarcomere organization
hair follicle placode formation
negative regulation of dendritic spine development
eyelid development in camera-type eye
fungiform papilla formation
cellular response to hydrogen peroxide
negative regulation of canonical Wnt signaling pathway
regulation of protein deacetylation
positive regulation of male mating behavior
negative regulation of stem cell population maintenance
positive regulation of stem cell population maintenance
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of stem cell differentiation
negative regulation of peptidyl-lysine acetylation
negative regulation of intrinsic apoptotic signaling pathway
regulation of embryonic development
response to amphetamine
cardiac muscle hypertrophy
response to xenobiotic stimulus
negative regulation of cell communication
negative regulation of signaling
response to caffeine
response to lipopolysaccharide
cellular response to heat
response to nicotine
response to cocaine
behavioral response to ethanol
response to hyperoxia
cellular response to retinoic acid
cellular response to transforming growth factor beta stimulus
response to alcohol
cellular response to dopamine
response to amyloid-beta
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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