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Invitrogen
Phospho-Dopamine Transporter (Thr53) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Dopamine Transporter (Thr53) Antibody (PA5-35414) in WB
Western blot of Dopamine Transporter in rat striatal lysate showing specific immunolabeling of a band at ~55 kDa corresponding to glycosylated form of the Phospho-Dopamine Transporter (Thr53) polyclonal antibody (Product # PA5-35414) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by pre-adsorption of the phosphopeptide used as the antigen, but not b... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of rat SLC6A3 conjugated to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.1mg/mL BSA, 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody contains enough material to conduct 10 mini-Western blots. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel.
Target Information
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DA transporter; DAT; dopamine transporter 1; solute carrier family 6 (neurotransmitter transporter), member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; unnamed protein product
Gene Aliases: DAT; Dat1
UniProt ID: (Mouse) Q61327, (Rat) P23977
Entrez Gene ID: (Mouse) 13162, (Rat) 24898
protease binding
receptor binding
neurotransmitter transporter activity
dopamine:sodium symporter activity
norepinephrine:sodium symporter activity
protein binding
monoamine transmembrane transporter activity
dopamine binding
amine binding
macromolecular complex binding
protein phosphatase 2A binding
heterocyclic compound binding
neurotransmitter uptake
neurotransmitter transport
amino acid transport
lactation
sensory perception of smell
locomotory behavior
response to xenobiotic stimulus
response to iron ion
adenohypophysis development
response to nicotine
sodium ion transmembrane transport
positive regulation of multicellular organism growth
regulation of dopamine metabolic process
response to cocaine
dopamine biosynthetic process
dopamine catabolic process
response to leptin
response to ethanol
cognition
dopamine uptake involved in synaptic transmission
response to cAMP
norepinephrine uptake
prepulse inhibition
dopamine uptake
hyaloid vascular plexus regression
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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