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Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody

View all (10) FOXO1/FOXO3/FOXO4 antibodies

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Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody

Product Details

PA5-36737

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Threonine 24
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553692

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 70-82 kDa.

Purity is >95% by SDS-PAGE.

Target Information

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AFX; AFX1; fork head domain transcription factor AFX1; forkhead box O3A; forkhead box protein O1A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOX04b; FOXO3A-; MGC120490; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; unnamed protein product

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Gene Aliases: AF6q21; AFX; AFX1; FKH1; FKHR; FKHRL1; FKHRL1P2; FOXO1A; FOXO2; FOXO3A; MLLT7

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UniProt ID: (Human) Q12778, (Human) O43524, (Human) P98177

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Entrez Gene ID: (Human) 2308, (Human) 2309, (Human) 4303

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding protein phosphatase 2A binding promoter-specific chromatin binding transcription regulatory region sequence-specific DNA binding transcription cofactor binding protein kinase binding mitochondrial RNA polymerase binding promoter specificity activity sequence-specific double-stranded DNA binding enzyme binding identical protein binding DNA-binding transcription factor binding
Process(es)
regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to oxidative stress negative regulation of cell proliferation insulin receptor signaling pathway positive regulation of smooth muscle cell migration negative regulation of angiogenesis response to nutrient levels positive regulation of transcription from RNA polymerase II promoter stem cell differentiation negative regulation of smooth muscle cell differentiation negative regulation of G0 to G1 transition response to water-immersion restraint stress negative regulation of transcription from RNA polymerase II promoter temperature homeostasis cellular glucose homeostasis protein acetylation apoptotic process cellular response to DNA damage stimulus cellular response to starvation positive regulation of autophagy cellular response to insulin stimulus negative regulation of stress-activated MAPK cascade positive regulation of smooth muscle cell apoptotic process cellular response to oxidative stress positive regulation of apoptotic process negative regulation of apoptotic process fat cell differentiation negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of insulin secretion regulation of transcription initiation from RNA polymerase II promoter cellular response to cold response to fatty acid cellular response to hyperoxia cellular response to nitric oxide energy homeostasis negative regulation of cardiac muscle hypertrophy in response to stress transcription from mitochondrial promoter regulation of translation response to xenobiotic stimulus positive regulation of muscle atrophy negative regulation of cell migration tumor necrosis factor-mediated signaling pathway cellular response to glucose starvation response to starvation positive regulation of neuron apoptotic process positive regulation of regulatory T cell differentiation positive regulation of erythrocyte differentiation negative regulation of neuron differentiation cellular response to glucose stimulus cellular response to corticosterone stimulus response to dexamethasone positive regulation of hydrogen peroxide-mediated programmed cell death positive regulation of pri-miRNA transcription from RNA polymerase II promoter cellular response to beta-amyloid cellular response to nerve growth factor stimulus
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