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Invitrogen
Phospho-C/EBP epsilon (Thr74) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-C/EBP epsilon (Thr74) Antibody (PA5-37522) in WB
Western Blot analysis of Phospho-C/EBP epsilon (Thr74) was performed using HUVEC cells treated with UV. The lane on the right is treated with the antigen-specific peptide. The membrane was probed with Phospho-C/EBP epsilon (Thr74) Polyclonal Antibody (Product # PA5-37522). .
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-37522
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around phosphorylation site of threonine 174 (L-A-T(p)-A-A) derived from Human C/EBP-epsilon
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control for Western blot is HUVEC cells.
Target Information
C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma, C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C EBP; CCAAT/enhancer binding protein (C/EBP), epsilon
Gene Aliases: c/EBP epsilon; C/EBP-epsilon; CRP1; IMD108; SGD1
UniProt ID: (Human) Q15744
Entrez Gene ID: (Human) 1053
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
macromolecular complex binding
sequence-specific double-stranded DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
defense response
myeloid cell differentiation
granulocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
integrated stress response signaling
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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