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Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody

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Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody

Product Details

PA5-37817

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of threonine 24/32(S-C-T(p)-W-P) derived from Human FOXO1/3/4-pan.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2554425

Product Specific Information

A suggested positive control for Western blot is 293 cells.

Target Information

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AFX; AFX1; fork head domain transcription factor AFX1; forkhead box O1a; forkhead box O3A; forkhead box protein O1A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma; forkhead in rhabdomyosarcoma-like 1; forkhead protein; forkhead protein 1; forkhead protein FKHR2; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOX04b; FOXO3A-; member of FKHR-subclass forkhead/winged helix family; MGC120490; myeloid/lymphoid or mixed lineage-leukemia translocation to 7 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; unnamed protein product

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Gene Aliases: 1110048B16Rik; 2010203A17Rik; AF6q21; AFX; AFX1; Afxh; FKH1; FKHR; Fkhr1; Fkhr2; FKHRL1; FKHRL1P2; FOXO1A; FOXO2; FOXO3A; MLLT7

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UniProt ID: (Human) Q12778, (Human) O43524, (Human) P98177, (Mouse) Q9R1E0, (Mouse) Q9WVH4, (Mouse) Q9WVH3

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Entrez Gene ID: (Human) 2308, (Human) 2309, (Human) 4303, (Mouse) 56458, (Mouse) 56484, (Mouse) 54601

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding protein phosphatase 2A binding promoter-specific chromatin binding transcription regulatory region sequence-specific DNA binding transcription cofactor binding protein kinase binding mitochondrial RNA polymerase binding promoter specificity activity sequence-specific double-stranded DNA binding enzyme binding identical protein binding DNA-binding transcription factor binding transcription coactivator binding transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovulation from ovarian follicle initiation of primordial ovarian follicle growth antral ovarian follicle growth oocyte maturation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from mitochondrial promoter regulation of translation phagocytosis response to xenobiotic stimulus gene expression positive regulation of autophagy positive regulation of muscle atrophy DNA damage response, signal transduction by p53 class mediator negative regulation of cell migration response to nutrient levels tumor necrosis factor-mediated signaling pathway cellular response to oxidative stress cellular response to glucose starvation glucose homeostasis response to starvation positive regulation of apoptotic process positive regulation of neuron apoptotic process positive regulation of regulatory T cell differentiation positive regulation of erythrocyte differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter brain morphogenesis response to fatty acid cellular response to glucose stimulus cellular response to corticosterone stimulus response to dexamethasone negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance extrinsic apoptotic signaling pathway in absence of ligand positive regulation of hydrogen peroxide-mediated programmed cell death positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of reactive oxygen species biosynthetic process cellular response to beta-amyloid cellular response to nerve growth factor stimulus response to water-immersion restraint stress regulation of neural precursor cell proliferation positive regulation of endothelial cell apoptotic process regulation of reactive oxygen species metabolic process response to oxidative stress negative regulation of cell proliferation insulin receptor signaling pathway positive regulation of smooth muscle cell migration negative regulation of angiogenesis stem cell differentiation negative regulation of smooth muscle cell differentiation negative regulation of G0 to G1 transition temperature homeostasis cellular glucose homeostasis protein acetylation apoptotic process cellular response to DNA damage stimulus cellular response to starvation cellular response to insulin stimulus negative regulation of stress-activated MAPK cascade positive regulation of smooth muscle cell apoptotic process negative regulation of apoptotic process fat cell differentiation negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of protein catabolic process negative regulation of transcription, DNA-templated negative regulation of insulin secretion regulation of transcription initiation from RNA polymerase II promoter cellular response to cold cellular response to hyperoxia cellular response to nitric oxide energy homeostasis negative regulation of cardiac muscle hypertrophy in response to stress blood vessel development gluconeogenesis regulation of gluconeogenesis response to insulin regulation of cell proliferation enamel mineralization cellular response to hydrogen peroxide cellular response to dexamethasone stimulus response to fluoride mitotic G2 DNA damage checkpoint proteasome-mediated ubiquitin-dependent protein catabolic process protein K48-linked ubiquitination
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