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Invitrogen

Phospho-RIP2 (Ser176) Polyclonal Antibody

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Phospho-RIP2 (Ser176) Polyclonal Antibody

Product Details

PA5-40164

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of serine 176 (S-L-S(p)-Q-S) derived from Human RIPK2.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610276

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CARD domain protein; phosphorylated protein; CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARDIAK; RIP-like kinase; EC 2.7.11.1; growth-in; growth-inhibiting gene 30; kinase RIPK2; protein kinase; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; receptor-interacting protein 2; RIP 2; RIP-2; RIP-like; RIP2; serine/threonine kinase homolog; contains CARD motif; tyrosine-protein kinase RIPK2; unnamed protein product; WUGSC:H_RG437L15.1

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Gene Aliases: 2210420D18Rik; CARD3; CARDIAK; CCK; D4Bwg0615e; GIG30; RICK; RIP2

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UniProt ID: (Human) O43353, (Mouse) P58801

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Entrez Gene ID: (Human) 8767, (Mouse) 192656

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Function(s)
protein kinase activity protein serine/threonine kinase activity JUN kinase kinase kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding LIM domain binding signaling adaptor activity identical protein binding protein homodimerization activity CARD domain binding caspase binding protein serine kinase activity
Process(es)
positive regulation of cytokine production positive regulation of cytokine-mediated signaling pathway adaptive immune response positive regulation of T-helper 1 type immune response apoptotic process signal transduction I-kappaB kinase/NF-kappaB signaling positive regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade positive regulation of immature T cell proliferation toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway p38MAPK cascade T cell proliferation defense response to bacterium regulation of apoptotic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA innate immune response positive regulation of T-helper 1 cell differentiation positive regulation of JNK cascade positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-positive bacterium T cell receptor signaling pathway protein homooligomerization positive regulation of ERK1 and ERK2 cascade nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway response to interleukin-1 response to interleukin-12 response to interleukin-18 cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to muramyl dipeptide protein linear polyubiquitination cell surface toll-like receptor signaling pathway positive regulation of protein K63-linked ubiquitination positive regulation of xenophagy MAPK cascade inflammatory response positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of transcription from RNA polymerase II promoter
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