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Invitrogen
CX3CL1 Polyclonal Antibody
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FIGURE: 1 / 2
CX3CL1 Antibody (PA5-47728) in IHC (F)
Immunohistochemical analysis of CX3CL1 in perfusion fixed frozen sections of mouse brain (cingulate cortex). Samples were incubated in CX3CL1 polyclonal antibody (Product # PA5-47728) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-47728
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant rat CX3CL1/Fractalkine Gln25-Gly100
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blots, less than 5% cross-reactivity with recombinant human (rh) Eotaxin is observed. This antibody neutralizes the biological activity of rhCX3CL1 and rmCX3CL1 with similar effectiveness.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; neurotactin; small inducible cytokine subfamily D, 1; small-inducible cytokine D1
Gene Aliases: Cx3c; Scyd1
UniProt ID: (Rat) O55145
Entrez Gene ID: (Rat) 89808
response to hypoxia
microglial cell activation
positive regulation of neuroblast proliferation
leukocyte migration involved in inflammatory response
response to ischemia
chemotaxis
inflammatory response
immune response
cell adhesion
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
positive regulation of macrophage chemotaxis
positive regulation of neuron projection development
cytokine-mediated signaling pathway
positive regulation of cell migration
negative regulation of cell migration
neutrophil chemotaxis
regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of actin filament bundle assembly
negative regulation of interleukin-1 alpha production
negative regulation of tumor necrosis factor production
positive regulation of transforming growth factor beta1 production
integrin activation
autocrine signaling
wound healing
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
positive regulation of angiogenesis
positive regulation of cell adhesion
negative regulation of vasoconstriction
positive regulation of transcription from RNA polymerase II promoter
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
positive chemotaxis
positive regulation of calcium-independent cell-cell adhesion
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
angiogenesis involved in wound healing
inhibitory postsynaptic potential
cell chemotaxis
microglial cell proliferation
antimicrobial humoral immune response mediated by antimicrobial peptide
neuron cellular homeostasis
chemokine-mediated signaling pathway
cellular response to tumor necrosis factor
cell-cell adhesion
negative regulation of hippocampal neuron apoptotic process
negative regulation of glutamate receptor signaling pathway
negative regulation of microglial cell activation
positive regulation of microglial cell migration
negative regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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