Search
Invitrogen
HOXA5 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
HOXA5 Antibody (PA5-50045) in ICC/IF
Immunofluorescent analysis of HoxA5 (red) in HUES3::Hb9-GFP human embryonic stem cells patterned to cervical neuromesodermal progenitors as previously described (Lippmann et. al. 2015) and subsequently differentiated into motor neurons (green) using smoothened agonist, purmorphamine and retinoic acid. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 5% normal donkey serum in tris buffered saline containing 0.3% Triton-X (TBSDT) for at least one hour at 4C. Cells were stained with a monoclonal HoxA5 antibody (Pr... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-50045
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from N-terminal of human HXA5.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total HOXA5 protein.
This antibody detects HoxA5 in cervical neuromesodermal progenitors.
Target Information
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA5 gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: homeo box 1C; homeo box A5; homeo domain protein; homeobox protein Hox-1.3; homeobox protein Hox-1C; homeobox protein Hox-A5-like; homeobox protein HOXA5; homeobox protein M2; hox-1.3; Hox-1C; HOXA5; unnamed protein product
Gene Aliases: Hox-1.3; HOX1; HOX1.3; HOX1C
UniProt ID: (Human) P20719, (Mouse) P09021
Entrez Gene ID: (Human) 3202, (Rat) 79241, (Mouse) 15402
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
anterior/posterior pattern specification
positive regulation of gene expression
negative regulation of angiogenesis
positive regulation of apoptotic process
positive regulation of myeloid cell differentiation
negative regulation of erythrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
skeletal system development
morphogenesis of an epithelium
respiratory system process
cell-cell signaling
pattern specification process
respiratory gaseous exchange
cell migration
lung development
thyroid gland development
regulation of mammary gland epithelial cell proliferation
multicellular organism growth
lung alveolus development
embryonic skeletal system morphogenesis
embryonic skeletal system development
bronchiole development
trachea morphogenesis
epithelial tube branching involved in lung morphogenesis
lung goblet cell differentiation
lobar bronchus epithelium development
lung-associated mesenchyme development
trachea cartilage morphogenesis
cartilage morphogenesis
intestinal epithelial cell maturation
mesenchymal-epithelial cell signaling
mammary gland epithelial cell differentiation
mammary gland alveolus development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support