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FIGURE: 1 / 13
RAB27B Antibody (PA5-54096) in ICC/IF
Immunofluorescence of RAB27B was performed using U-87 MG WT and RAB27B KD cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KD cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were sta... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-54096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human RAB27B. Recombinant protein control fragment (Product #RP-91715).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FETSAATGQN VEKAVETLLD LIMKRMEQCV EKTQIPDTVN GGNSGNLDGE KPPEKKCIC
Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 86%.
Target Information
RAB27B may be involved in targeting uroplakins to urothelial apical membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: unnamed protein product
Gene Aliases: C25KG
UniProt ID: (Human) O00194
Entrez Gene ID: (Human) 5874
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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