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Invitrogen
ASCL1 Polyclonal Antibody
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FIGURE: 1 / 8
ASCL1 Antibody (PA5-77868) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ASCL1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASCL1 Polyclonal Antibody (Product # PA5-77868) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-77868
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ASCL1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.65 mg/mL
Amount
165 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, SH-SY5Y, SH-SY5Y nuclear extract, PC-12, Rat2
Predicted Reactivity: Mouse (84%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like 1; achaete-scute complex-like protein 1; ASH-1; class A basic helix-loop-helix protein 46; KMT2H; mammalian achaete scute homolog 1; unnamed protein product
Gene Aliases: ASH1; bHLHa46; HASH1; MASH1
UniProt ID: (Human) P50553, (Mouse) Q02067, (Rat) P19359
Entrez Gene ID: (Dog) 482628, (Human) 429, (Mouse) 17172, (Rat) 64186
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
bHLH transcription factor binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
noradrenergic neuron development
noradrenergic neuron fate commitment
regulation of transcription from RNA polymerase II promoter
Notch signaling pathway
nervous system development
sensory organ development
heart development
regulation of gene expression
forebrain neuron differentiation
cerebral cortex GABAergic interneuron differentiation
neurogenesis
neuron differentiation
response to retinoic acid
negative regulation of apoptotic process
negative regulation of neuron differentiation
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
sympathetic nervous system development
neuron fate commitment
neuron fate specification
neuron development
regulation of neurogenesis
response to folic acid
lung epithelial cell differentiation
ventral spinal cord interneuron fate commitment
sympathetic ganglion development
response to epidermal growth factor
cellular response to magnetism
neuron migration
regulation of mitotic cell cycle
pattern specification process
neuroblast fate determination
neuroblast proliferation
regulation of Notch signaling pathway
glial cell differentiation
oligodendrocyte development
spinal cord association neuron differentiation
spinal cord oligodendrocyte cell differentiation
spinal cord oligodendrocyte cell fate specification
vestibular nucleus development
oligodendrocyte cell fate commitment
commitment of neuronal cell to specific neuron type in forebrain
central nervous system neuron development
cerebral cortex development
cell differentiation
regulation of epithelial cell differentiation
regulation of cell proliferation
positive regulation of neuron apoptotic process
regulation of cell differentiation
positive regulation of Notch signaling pathway
positive regulation of cell cycle
cell maturation
enteric nervous system development
parasympathetic nervous system development
generation of neurons
oligodendrocyte differentiation
positive regulation of neurogenesis
musculoskeletal movement, spinal reflex action
subpallium neuron fate commitment
regulation of timing of subpallium neuron differentiation
olfactory pit development
lung neuroendocrine cell differentiation
stomach neuroendocrine cell differentiation
carotid body glomus cell differentiation
adrenal chromaffin cell differentiation
positive regulation of neural precursor cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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