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Invitrogen
Cdc25A Polyclonal Antibody
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FIGURE: 1 / 5
Cdc25A Antibody (PA5-77902) in IHC (P)
Cdc25A Polyclonal Antibody detects CDC25A protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. Cdc25A Polyclonal Antibody (Product # PA5-77902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-77902
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human CDC25A. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: H1299, MCF-7
Predicted Reactivity: Dog (89%), Bovine (90%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CDC25 homolog A; CDC25A2; dual specificity phosphatase CDC25A; EC 3.1.3.48; MPIP1; phosphatase; putative; unnamed protein product
Gene Aliases: CDC25A2; D9Ertd393e
UniProt ID: (Human) P30304, (Rat) P48965, (Mouse) P48964
Entrez Gene ID: (Human) 993, (Rat) 171102, (Mouse) 12530
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
cell proliferation
response to radiation
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of DNA replication
positive regulation of G2/MI transition of meiotic cell cycle
positive regulation of cell cycle G2/M phase transition
positive regulation of cholangiocyte proliferation
nuclear envelope organization
mitotic nuclear envelope disassembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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