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Invitrogen
ASIC3 Polyclonal Antibody
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FIGURE: 1 / 2
ASIC3 Antibody (PA5-78825) in WB
Western blot analysis of ASIC3 in Lane 1: rat brain tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: U87 cell lysate, Lane 4: NEURO cell lysate, Lane 5: SMMC cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with ASIC3 polyclonal antibody (Product... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78825
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3 (56-73aa FLYQVAERVRYYREFHHQ).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Brain Tissue, Rat Testis Tissue, U87 Cell, NEURO Cell, SMMC Cell. Flow: U937 cell.
Target Information
ACCN3 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. ACCN3 encodes a member that is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acid sensing (proton gated) ion channel 3; acid-sensing (proton-gated) ion channel 3; amiloride-sensitive cation channel 3; amiloride-sensitive cation channel 3, testis; ASIC3; ASIC3b; ASIC3c; dorsal root ASIC; DRASIC; hASIC3; ion channel; modulatory subunit of ASIC2a; neuronal amiloride-sensitive cation channel 3; proton gated cation channel DRASIC; proton-gated cation channel subunit; testis sodium channel 1; unnamed protein product
Gene Aliases: ACCN3; DRASIC; SLNAC1; TNAC1
UniProt ID: (Human) Q9UHC3, (Mouse) Q6X1Y6, (Rat) O35240
Entrez Gene ID: (Human) 9311, (Mouse) 171209, (Rat) 286920
cation transport
sodium ion transport
response to heat
response to mechanical stimulus
response to acidic pH
calcium-mediated signaling
ion transmembrane transport
sodium ion transmembrane transport
detection of chemical stimulus involved in sensory perception
sensory perception of sour taste
detection of temperature stimulus involved in sensory perception
detection of temperature stimulus involved in sensory perception of pain
detection of mechanical stimulus involved in sensory perception of pain
detection of chemical stimulus involved in sensory perception of pain
detection of mechanical stimulus involved in sensory perception
membrane depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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