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Invitrogen
BAG3 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78855
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Bag3 recombinant protein (Position: H100-N561).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human MCF-7 whole cell, human A431 whole cell, human 293T whole cell, human U251 whole cell, human U87 whole cell. IHC: human lung cancer tissue.
Target Information
BAG3 are among BAG proteins that compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl-2 interacting cell death suppressor; bcl-2-binding protein Bis; Bcl-2-binding protein BIS transcript 2; BCL2 associated athanogene 3; BCL2-binding athanogene 3; DKFZp434E0610; docking protein CAIR-1; MGC104307; unnamed protein product
Gene Aliases: BAG-3; BIS; CAIR-1; CMD1HH; CMT2JJ; HMND15; MFM6
UniProt ID: (Human) O95817
Entrez Gene ID: (Human) 9531
autophagosome assembly
protein folding
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of striated muscle cell apoptotic process
spinal cord development
cellular response to heat
cellular response to unfolded protein
positive regulation of protein import into nucleus
negative regulation of apoptotic process
muscle cell cellular homeostasis
positive regulation of protein export from nucleus
protein stabilization
chaperone-mediated autophagy
aggresome assembly
extrinsic apoptotic signaling pathway
protein transport along microtubule
negative regulation of establishment of protein localization to mitochondrion
positive regulation of aggrephagy
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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