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Invitrogen
CD154 (CD40 Ligand) Polyclonal Antibody
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CD154 (CD40 Ligand) Antibody (PA5-78984) in IHC (P)
Immunohistochemistry analysis of CD154 (CD40 Ligand) on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CD154 (CD40 Ligand) polyclonal antibody (Product# PA5-78984) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabb... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78984
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD40L (47-65aa HRRLDKIEDERNLHEDFVF).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: MCF-7 whole cell, HELA whole cell, JURKAT whole cell, HMY2 whole cell, COLO320 whole cell. IHC: rat intestine tissue, human mammary cancer tissue.
Target Information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; transmembrane protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor ligand superfamily member 5; unnamed protein product
Gene Aliases: CD154; CD40L; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
UniProt ID: (Human) P29965, (Rat) Q9Z2V2
Entrez Gene ID: (Human) 959, (Rat) 84349
regulation of immunoglobulin production
regulation of immune system process
positive regulation of immune response to tumor cell
inflammatory response
immune response
cell surface receptor signaling pathway
integrin-mediated signaling pathway
positive regulation of cell proliferation
regulation of gene expression
positive regulation of gene expression
CD40 signaling pathway
platelet activation
B cell differentiation
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-4 production
response to cobalamin
B cell proliferation
positive regulation of T cell proliferation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
isotype switching
positive regulation of MHC class II biosynthetic process
cellular response to tumor necrosis factor
dendritic cell differentiation
response to peptide
positive regulation of endothelial cell apoptotic process
positive regulation of dendritic cell differentiation
positive regulation of extrinsic apoptotic signaling pathway
cell communication
leukocyte cell-cell adhesion
signaling
T cell costimulation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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