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Invitrogen
ERAB Polyclonal Antibody
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FIGURE: 1 / 11
ERAB Antibody (PA5-79401) in ICC/IF
Immunocytochemistry analysis of ERAB using anti-ERAB antibody (Product # PA5-79401) . ERAB was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ERAB antibody (Product # PA5-79401) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79401
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ERAB recombinant protein (Position: E48-P261).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human 293T whole cell, human Hela whole cell, human MOLT4 whole cell. IHC: Human Mammary Cancer tissue. ICC/IF: U20S cell, Hela Cell, MCF-7 Cell, MCF-7 Cell, SMMC-7721 Cell. Flow: A549 cell, A431 cell.
Target Information
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; AB-binding alcohol dehydrogenase; amyloid-beta peptide binding alcohol dehydrogenase; endoplasmic reticulum-associated amyloid beta-peptide-binding protein; HADH2; HADH2 protein; hydroxysteroid (17-beta) dehydrogenase 10 isoform HSD17B10_1; hydroxysteroid (17-beta) dehydrogenase 10 isoform HSD17B10_2; mitochondrial ribo; mitochondrial ribonuclease P protein 2; mitochondrial RNas; mitochondrial RNase P subunit 2; RP3-339A18.2; short chain L-3-hydroxyacyl-CoA dehydrogenase type 2; short chain type dehydrogenase/reductase XH98G2; type 10 17b-HSD; X-linked alcohol dehydrogenase
Gene Aliases: 17b-HSD10; ABAD; CAMR; DUPXp11.22; ERAB; HADH2; HCD2; HSD10MD; MHBD; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1
UniProt ID: (Human) Q99714
Entrez Gene ID: (Human) 3028
tRNA binding
RNA binding
3-hydroxyacyl-CoA dehydrogenase activity
estradiol 17-beta-dehydrogenase activity
protein binding
cholate 7-alpha-dehydrogenase activity
long-chain-3-hydroxyacyl-CoA dehydrogenase activity
17-beta-hydroxysteroid dehydrogenase (NAD+) activity
3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity
testosterone dehydrogenase (NAD+) activity
androstan-3-alpha,17-beta-diol dehydrogenase activity
chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
isoursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
ursodeoxycholate 7-beta-dehydrogenase (NAD+) activity
cardiolipin dehydrogenase (NAD+) activity
tRNA 3'-terminal CCA addition
isoleucine catabolic process
lipid metabolic process
fatty acid metabolic process
fatty acid beta-oxidation
bile acid biosynthetic process
mitochondrion organization
C21-steroid hormone metabolic process
androgen metabolic process
estrogen metabolic process
tRNA 3'-end processing
protein homotetramerization
brexanolone metabolic process
mitochondrial tRNA methylation
mitochondrial tRNA 5'-end processing
mitochondrial tRNA 3'-end processing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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