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Invitrogen
IL1F6 Polyclonal Antibody
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FIGURE: 1 / 2
IL1F6 Antibody (PA5-79501) in IHC (P)
Immunohistochemistry analysis of IL1F6 on paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with IL1F6 polyclonal antibody (Product# PA5-79501) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human IL36 alpha recombinant protein (Position: K6-D151).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue, human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human HepG2 whole cell. IHC: human intestinal cancer tissue.
Target Information
The IL-1 family is comprised of 11 structurally related ligands, including recently re-named IL-36alpha (IL-1F6), beta (IL-1F8), and gamma (IL-1F9). IL-36alpha is highly expressed in psoriatic plaques, as are IL-36beta and IL-36gamma, and is the only IL-1 family member found to be expressed by T cells, as well as monocytes and B cells. Primarily found in skin and lymphoid tissues, IL-36alpha is also found to a lesser extent in tissues of the fetal brain, trachea, stomach, and intestine. IL-36alpha signals through a combination of the IL-1Rrp2 (IL-1R6) receptor, which is primarily expressed on certain dendritic cells, and the widely expressed IL-RAcP (IL-1 receptor accessory protein). IL-36alpha also functions as an agonist of NF-kappaB and MAPK pathways, and induces the production of proinflammatory cytokines and chemokines, including IL-6, IL-8, BD-2, and BD-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; mIL-1H1; FIL1 epsilon; Fil1epsilon; IL-1 epsilon; IL-1F6; IL-1F6 (FIL-1-epsilon); Interleukin; interleukin 1; interleukin 1 family, member 6; interleukin 1 family, member 6 (epsilon); interleukin 1 family, member 9; interleukin-1 epsilon; interleukin-1 family member 6; interleukin-1 homolog 1; Interleukin-36; similar to IL-1; unnamed protein product
Gene Aliases: FIL1; FIL1(EPSILON); FIL1E; If36a; IL-1F6; IL-1H1; IL1(EPSILON); IL1F6; Il1f9; IL1RP2
UniProt ID: (Human) Q9UHA7, (Mouse) Q9JLA2
Entrez Gene ID: (Human) 27179, (Mouse) 54448, (Rat) 296541
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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