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Invitrogen
NLRC4 Polyclonal Antibody
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FIGURE: 1 / 3
NLRC4 Antibody (PA5-79739) in ICC/IF
Immunofluorescence analysis of NLRC4 (NLR family CARD domain-containing protein 4) was performed using log phase THP-1 and Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NLRC4 Polyclonal Antibody (Product # PA5-79739) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79739
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12 (838-874aa KILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDR).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: 22RV1 whole cell, RH35 whole cell, NIH3T3 whole cell.
Target Information
NLRC4 encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CARD protein; CARD, LRR, and NACHT-containing protein; CARD12; CARD12; CED4/Apaf-1 family member; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CED-4-like protein Clan; ice protease-activating factor; ICE-protease activating factor; IPAF; CED4/Apaf-1 family member; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; unnamed protein product
Gene Aliases: 9530011P19Rik; AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
UniProt ID: (Human) Q9NPP4, (Rat) F1M649, (Mouse) Q3UP24
Entrez Gene ID: (Human) 58484, (Rat) 298784, (Mouse) 268973
activation of innate immune response
inflammatory response
positive regulation of protein processing
detection of bacterium
positive regulation of interleukin-1 beta production
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
pyroptosis
pattern recognition receptor signaling pathway
icosanoid biosynthetic process
positive regulation of inflammatory response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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