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NUCB2 Polyclonal Antibody
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FIGURE: 1 / 3
NUCB2 Antibody (PA5-79767) in IHC (P)
Immunohistochemistry analysis of NUCB2 on paraffin-embedded mouse gastric tissue. Sample was incubated with NUCB2 polyclonal antibody (Product# PA5-79767) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79767
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HEPA whole cell. IHC: mouse gaster tissue, rat gaster tissue.
Target Information
NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-binding protein NEFA; nesfatin-1; Nucleobindin-2/Nesfatin-1; prepronefastin; prepronesfatin; unnamed protein product
Gene Aliases: Calnuc; Nefa; Nesfatin-1; p54
UniProt ID: (Mouse) P81117, (Rat) Q9JI85
Entrez Gene ID: (Mouse) 53322, (Rat) 59295
cellular calcium ion homeostasis
small GTPase mediated signal transduction
negative regulation of appetite
negative regulation of fat cell differentiation
positive regulation of fatty acid oxidation
negative regulation of insulin receptor signaling pathway
negative regulation of glucagon secretion
negative regulation of cAMP/PKA signal transduction
insulin metabolic process
positive regulation of testosterone secretion
response to glucose
response to starvation
response to human chorionic gonadotropin
response to melanocyte-stimulating hormone
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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