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Invitrogen
PF4 Polyclonal Antibody
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FIGURE: 1 / 2
PF4 Antibody (PA5-79811) in IHC (P)
Immunohistochemistry (Paraffin) analysis of PF4 in paraffin-embedded section of mouse lung cancer tissue using PF4 Polyclonal Antibody (Product # PA5-79811). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79811
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse PF4 recombinant protein (Position: V30-S105).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse spleen tissue. IHC: mouse lung cancer tissue.
Target Information
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; unnamed protein product
Gene Aliases: Cxcl4; Scyb4
UniProt ID: (Mouse) Q9Z126
Entrez Gene ID: (Mouse) 56744
chemotaxis
defense response
inflammatory response
immune response
adenylate cyclase-activating G-protein coupled receptor signaling pathway
negative regulation of angiogenesis
cytokine-mediated signaling pathway
platelet activation
neutrophil chemotaxis
leukocyte chemotaxis
regulation of cell proliferation
defense response to protozoan
negative regulation of megakaryocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
antimicrobial humoral immune response mediated by antimicrobial peptide
protein-containing complex assembly
cellular response to lipopolysaccharide
response to aldosterone
negative regulation of T-helper 17 cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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