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Invitrogen
CPI-17 Polyclonal Antibody
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FIGURE: 1 / 9
CPI-17 Antibody (PA5-79858) in ICC/IF
Immunocytochemistry analysis of CPI17 alpha using anti-CPI17 alpha antibody (Product # PA5-79858) . CPI17 alpha was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CPI17 alpha antibody (Product # PA5-79858) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was cou... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79858
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue, PANC-1 whole cell. IHC: mouse testis tissue, rat lung tissue, human intestinal cancer tissue, human lung cancer tissue, human placenta tissue. ICC/IF: U20S cell. Flow: U20S cell.
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; CPI; hCPI; mCPI; PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of 17 kDa; rCPI
Gene Aliases: 1110001M11Rik; CPI-17; CPI17; PPP1INL
UniProt ID: (Human) Q96A00, (Mouse) Q91VC7, (Rat) Q99MC0
Entrez Gene ID: (Human) 94274, (Mouse) 68458, (Rat) 114004
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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