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Invitrogen
PKC eta Polyclonal Antibody
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FIGURE: 1 / 4
PKC eta Antibody (PA5-79871) in WB
Western blot analysis of PKC eta in Lane 1: A431 whole cell lysate, Lane 2: A549 whole cell lysate, Lane 3: HeLa whole cell lysate, Lane 4: SKOV whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with PKC eta polyclonal antibody (Product # PA5-79871)... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PKC eta recombinant protein (Position: R30-D389).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A431 whole cell, A549 whole cell, HELA whole cell, SKOV whole cell. Flow: A431 cell, K562 cell, MCF-7 cell.
Target Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC26269; MGC5363; Protein uPEP2; unnamed protein product
Gene Aliases: nPKC-eta; PKC-L; PKCL; PRKCL; uORF2
UniProt ID: (Human) P24723
Entrez Gene ID: (Human) 5583
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
calcium-independent protein kinase C activity
protein binding
ATP binding
enzyme binding
protein serine/threonine kinase inhibitor activity
small GTPase binding
translation regulator activity
protein serine kinase activity
protein serine/threonine kinase binding
protein phosphorylation
signal transduction
positive regulation of macrophage derived foam cell differentiation
negative regulation of translation
positive regulation of translation in response to stress
cellular response to amino acid starvation
negative regulation of glial cell apoptotic process
intracellular signal transduction
positive regulation of keratinocyte differentiation
positive regulation of B cell receptor signaling pathway
positive regulation of glial cell proliferation
phospholipase C/protein kinase C signal transduction
positive regulation of protein localization to plasma membrane
regulation of bicellular tight junction assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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