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Invitrogen
RPA70 Polyclonal Antibody
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FIGURE: 1 / 3
RPA70 Antibody (PA5-79933) in ICC/IF
Immunocytochemistry analysis of RPA70 using anti-RPA70 antibody (Product # PA5-79933) . RPA70 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-RPA70 antibody (Product # PA5-79933) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counte... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RPA70 (533-568aa QESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFR).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human HepG2 whole cell, human 293T whole cell. ICC/IF: A549 cell. Flow: U251 cell.
Target Information
This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MSTP075; replication factor A protein 1; replication protein A1, 70kDa; RF-A protein 1; RP-A p70; single-stranded DNA-binding protein; unnamed protein product
Gene Aliases: HSSB; MST075; PFBMFT6; REPA1; RF-A; RP-A; RPA70
UniProt ID: (Human) P27694
Entrez Gene ID: (Human) 6117
telomere maintenance
double-strand break repair via homologous recombination
DNA replication
DNA-dependent DNA replication
DNA repair
base-excision repair
nucleotide-excision repair
mismatch repair
DNA recombination
chromatin organization
cellular response to DNA damage stimulus
telomere maintenance via telomerase
protein localization to chromosome
meiotic cell cycle
protein localization to site of double-strand break
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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