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Invitrogen
RANK (CD265) Polyclonal Antibody
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FIGURE: 1 / 3
RANK (CD265) Antibody (PA5-80144) in IHC (P)
Immunohistochemistry analysis of RANK (CD265) on paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with RANK (CD265) polyclonal antibody (Product# PA5-80144) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-80144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human RANK (235-262aa YRKKGKALTANLWHWINEACGRLSGDKE).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat thymus tissue, mouse thymus tissue, HEPG2 whole cell. IHC: human intestinal cancer tissue, human lung cancer tissue.
Target Information
RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: familial expansile osteolysis; loss of heterozygosity, 18, chromosomal region 1; osteoclast differentiation factor receptor; Paget disease of bone 2; receptor activator of NF-kappaB; receptor activator of NF-KB; receptor activator of nuclear factor kappa B; receptor activator of nuclear factor-kappa B; TRANCE receptor; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Gene Aliases: CD265; FEO; LOH18CR1; Ly109; mRANK; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; RGD1563614; TRANCE-R; TRANCER
UniProt ID: (Human) Q9Y6Q6, (Mouse) O35305
Entrez Gene ID: (Human) 8792, (Rat) 498206, (Mouse) 21934
ossification
adaptive immune response
immune system process
monocyte chemotaxis
signal transduction
cell-cell signaling
positive regulation of cell proliferation
response to mechanical stimulus
osteoclast differentiation
response to lipopolysaccharide
response to insulin
tumor necrosis factor-mediated signaling pathway
cellular response to zinc ion starvation
response to tumor necrosis factor
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to ethanol
positive regulation of osteoclast differentiation
positive regulation of bone resorption
positive regulation of JNK cascade
lymph node development
circadian temperature homeostasis
positive regulation of ERK1 and ERK2 cascade
response to interleukin-1
positive regulation of fever generation by positive regulation of prostaglandin secretion
multinuclear osteoclast differentiation
positive regulation of NIK/NF-kappaB signaling
mammary gland alveolus development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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