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Invitrogen
14-3-3 sigma Polyclonal Antibody
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FIGURE: 1 / 3
14-3-3 sigma Antibody (PA5-81018) in ICC/IF
Immunofluorescence staining of 14-3-3 sigma in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with 14-3-3 sigma Polyclonal Antibody (Product # PA5-81018, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positiv... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-81018
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human 14-3-3 sigma/Stratifin/YWHAS Protein (Met1-Ser248)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human 14-3-3 sigma/Stratifin/YWHAS.
Target Information
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3sigma; epithelial cell marker protein 1; HGNC:10773; SFN
Gene Aliases: YWHAS
UniProt ID: (Human) P31947
Entrez Gene ID: (Human) 2810
release of cytochrome c from mitochondria
negative regulation of protein kinase activity
protein export from nucleus
signal transduction
protein localization
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of epidermal cell division
regulation of cell-cell adhesion
keratinocyte differentiation
positive regulation of cell growth
regulation of protein localization
positive regulation of hippo signaling
positive regulation of epidermal cell differentiation
positive regulation of keratinocyte differentiation
positive regulation of cell adhesion
negative regulation of innate immune response
positive regulation of protein export from nucleus
establishment of skin barrier
cAMP/PKA signal transduction
negative regulation of protein localization to plasma membrane
positive regulation of cellular protein localization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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