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Invitrogen
RAD51 Polyclonal Antibody
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FIGURE: 1 / 3
RAD51 Antibody (PA5-81248) in ICC/IF
Immunofluorescence staining of RAD51 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with RAD51 Polyclonal Antibody (Product # PA5-81248, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-81248
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human RAD51
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human RAD51.
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; RAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A; unnamed protein product
Gene Aliases: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51A; RECA
UniProt ID: (Human) Q06609
Entrez Gene ID: (Human) 5888
recombinase activity
nucleotide binding
DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
protein binding
ATP binding
DNA-dependent ATPase activity
ATPase activity
enzyme binding
nucleosome binding
identical protein binding
DNA polymerase binding
ATP-dependent DNA damage sensor activity
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA metabolic process
DNA repair
DNA recombination
mitotic recombination
cellular response to DNA damage stimulus
meiosis I
reciprocal meiotic recombination
response to xenobiotic stimulus
response to toxic substance
response to X-ray
regulation of double-strand break repair via homologous recombination
telomere maintenance via telomere lengthening
replication fork processing
interstrand cross-link repair
strand invasion
meiotic cell cycle
chromosome organization involved in meiotic cell cycle
cellular response to ionizing radiation
cellular response to gamma radiation
cellular response to cisplatin
cellular response to camptothecin
response to glucoside
mitotic recombination-dependent replication fork processing
double-strand break repair involved in meiotic recombination
regulation of DNA damage checkpoint
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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