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Invitrogen
alanyl-tRNA Synthetase Polyclonal Antibody
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FIGURE: 1 / 5
alanyl-tRNA Synthetase Antibody (PA5-81309) in ICC/IF
Immunocytochemistry analysis of alanyl-tRNA Synthetase in HeLa cells using alanyl-tRNA Synthetase Polyclonal (Product # PA5-81309). Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-81309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Mouse AARS/alanyl-tRNA synthetase Protein (1-968).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Mouse AARS/alanyl-tRNA synthetase.
Target Information
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E. coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; alanyl-tRNA synthetase, cytoplasmic; AlaRS; Protein lactyltransferase AARS1; Protein sticky; Sti
Gene Aliases: Aars; Aars1; AI316495; C76919; sti
UniProt ID: (Mouse) Q8BGQ7
Entrez Gene ID: (Mouse) 234734
tRNA binding
nucleotide binding
aminoacyl-tRNA editing activity
nucleic acid binding
RNA binding
aminoacyl-tRNA ligase activity
alanine-tRNA ligase activity
protein binding
ATP binding
amino acid binding
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
metal ion binding
aminoacyl-tRNA synthetase
hair follicle development
tRNA modification
translation
alanyl-tRNA aminoacylation
regulation of translational fidelity
protein folding
cerebellar Purkinje cell layer development
endoplasmic reticulum unfolded protein response
cellular response to unfolded protein
tRNA aminoacylation
response to amino acid
negative regulation of neuron apoptotic process
skin development
neuromuscular process controlling balance
neuromuscular process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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