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Invitrogen

Phospho-CDK1 (Tyr15) Polyclonal Antibody

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View all (90) CDK1 antibodies

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Phospho-CDK1 (Tyr15) Polyclonal Antibody

Product Details

PA5-85508

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Fruit fly, Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr15 of human CDC2. The exact sequence is proprietary.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Amount

84 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792648

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Japanese Medaka (100%), Drosophila (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Positive Control: HeLa (untreated), HeLa (2mM double thymidine treatment and recovery for 3.5hr), HeLa (2mM double thymidine treatment and recovery for 12hr), HeLa (100 ng/mL Nocodazole treatment for 24hr), Rat-2 (untreated), Rat-2 (100 ng/mL Nocodazole treatment for 24.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ABL1; CDC2; cdc2 kinase; Cdk1-PA; cell cycle controller CDC2; cell division control; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; CG5363-PA; cyclin dependent kinase; cyclin-dependent kinase; DKFZp686L20222; KCIP-1; LD38718p; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; Protein 1054; protein 2; unnamed protein product

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Gene Aliases: cdc; 5363; CDC2; CDC28A; cdc2Dm; CDCDm; Cdk-1; CDK1; CDK1/CDC2; CG5363; Dcdc2; dCdk1; Dm cdc2; DmCdc2; DmCdk1; Dmel\CG5363; Dmel_CG5363; group 4; l(2)31Eh; P34CDC2

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UniProt ID: (Human) P06493, (Fruit fly) P23572

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Entrez Gene ID: (Human) 983, (Fruit fly) 34411

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Function(s)
chromatin binding protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity cyclin binding Hsp70 protein binding histone kinase activity cyclin-dependent protein kinase activity protein serine kinase activity non-receptor serine/threonine protein kinase
Process(es)
G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle microtubule cytoskeleton organization DNA replication DNA repair chromatin remodeling regulation of transcription from RNA polymerase II promoter cellular response to DNA damage stimulus mitotic nuclear envelope disassembly mitotic G2 DNA damage checkpoint centrosome cycle pronuclear fusion response to toxic substance positive regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle regulation of Schwann cell differentiation response to amine response to activity cell migration protein deubiquitination peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation negative regulation of protein ubiquitination protein localization to kinetochore positive regulation of protein import into nucleus response to hydrogen peroxide regulation of circadian rhythm negative regulation of apoptotic process response to ethanol positive regulation of DNA replication positive regulation of smoothened signaling pathway regulation of embryonic development response to cadmium ion response to copper ion response to axon injury ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation positive regulation of mitotic sister chromatid segregation protein-containing complex assembly cellular response to hydrogen peroxide ERK1 and ERK2 cascade Golgi disassembly regulation of heterochromatin organization positive regulation of protein localization to nucleus regulation of mitotic attachment of spindle microtubules to kinetochore microtubule cytoskeleton organization involved in mitosis positive regulation of mitochondrial ATP synthesis coupled electron transport positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process embryonic development via the syncytial blastoderm protein phosphorylation male meiosis spermatogonial cell division regulation of DNA-dependent DNA replication initiation ovarian follicle cell development regulation of protein localization mitotic G2/M transition checkpoint germarium-derived cystoblast division asymmetric neuroblast division regulation of cell cycle phase transition
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