Search
Invitrogen
RUNX2 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
RUNX2 Antibody (PA5-86506) in WB
Western blot analysis of RUNX2 in Lane 1: SK-OVCAR3 whole cell lysate (40 µg), Lane 2: Jurkat whole cell lysate (40 µg), Lane 3: SP2/0 whole cell lysate (40 µg), Lane 4: H9C2 whole cell lysate (40 µg). Sample was incubated with RUNX2 polyclonal antibody (Product # PA5-86506) using a 1:500 dilution.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-86506
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 246-297 of Human RUNX-2.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acute myeloid leukemia 3 protein; AKV core binding factor; AML-3; core binding factor alpha 1; core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; nuclear protein; oncogene AML-3; osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2 alpha A; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; transcription factor; transcription factor Cbfa1/Osf2
Gene Aliases: AML3; Cbf; CBF-alpha-1; Cbfa-1; CBFA1; CCD; CCD1; CLCD; LS3; OSF-2; OSF2; PEA2aA; Pebp2a1; PEBP2aA; Pebpa2a
UniProt ID: (Human) Q13950, (Mouse) Q08775
Entrez Gene ID: (Human) 860, (Mouse) 12393, (Rat) 367218
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ATP binding
sequence-specific double-stranded DNA binding
transcription regulatory region sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
protein domain specific binding
chromatin DNA binding
histone deacetylase binding
bHLH transcription factor binding
general transcription initiation factor binding
DNA-binding transcription factor binding
skeletal system development
ossification
osteoblast differentiation
endochondral ossification
osteoblast fate commitment
chondrocyte differentiation
chondrocyte development
osteoblast development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transmembrane receptor protein serine/threonine kinase signaling pathway
cell proliferation
positive regulation of cell proliferation
gene expression
regulation of gene expression
positive regulation of Schwann cell proliferation
positive regulation of gene expression
hemopoiesis
neuron differentiation
T cell differentiation
regulation of ossification
BMP signaling pathway
positive regulation of chondrocyte differentiation
embryonic forelimb morphogenesis
ligamentous ossification
regulation of fibroblast growth factor receptor signaling pathway
odontogenesis of dentin-containing tooth
regulation of odontogenesis of dentin-containing tooth
regulation of cell differentiation
regulation of osteoblast differentiation
positive regulation of osteoblast differentiation
positive regulation of ossification
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell development
cell maturation
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
stem cell differentiation
positive regulation of multicellular organismal process
SMAD protein signal transduction
response to sodium phosphate
regulation of multicellular organismal development
response to mechanical stimulus
response to insulin
cellular response to zinc ion starvation
cellular response to fibroblast growth factor stimulus
positive regulation of developmental process
cellular response to calcium ion
cellular response to parathyroid hormone stimulus
cellular response to hyperoxia
response to dexamethasone
dentinogenesis
bone regeneration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support