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Invitrogen
BMI-1 Polyclonal Antibody
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FIGURE: 1 / 3
BMI-1 Antibody (PA5-87371) in WB
Western blot analysis of BMI-1 in extracts of various cell lines. Samples were incubated with BMI-1 Polyclonal antibody (Product # PA5-87371) using a dilution of 1:5,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-87371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Bmi1 (NP_0051714)
Conjugate
Form
Liquid
Concentration
2.85 mg/mL
Amount
285 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LKDYYTLMDI AYIYTWRRNG PLPLKYRVRP TCKRMKISHQ RDGLTNAGEL ESDSGSDKAN SPAGGIPSTS SCLPSPSTPV QSPHPQFPHI SSTMNGTSNS P
Positive Samples: HT-1080, K-562, U-937; Cellular Location: Cytoplasm, Nucleus
Target Information
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; COMMD3; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; putative; RING finger protein 51; RP11-573G6.1
Gene Aliases: AW546694; Bmi-1; BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
UniProt ID: (Human) P35226, (Mouse) P25916
Entrez Gene ID: (Human) 648, (Rat) 307151, (Mouse) 12151
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
in utero embryonic development
DNA methylation
humoral immune response
brain development
regulation of gene expression
histone acetylation
histone ubiquitination
rostrocaudal neural tube patterning
hemopoiesis
positive regulation of B cell proliferation
positive regulation of immature T cell proliferation in thymus
somatic stem cell division
positive regulation of fibroblast proliferation
embryonic skeletal system morphogenesis
embryonic skeletal system development
regulation of catalytic activity
positive regulation of ubiquitin-protein transferase activity
cellular response to interleukin-1
cellular response to dexamethasone stimulus
negative regulation of apoptotic signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular process
chromatin modification
hematopoietic stem cell homeostasis
chromatin organization
chromatin remodeling
methylation-dependent chromatin silencing
segment specification
negative regulation of gene expression, epigenetic
regulation of kidney development
apoptotic signaling pathway
regulation of adaxial/abaxial pattern formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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