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Invitrogen
SERCA1 ATPase Polyclonal Antibody
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FIGURE: 1 / 1
SERCA1 ATPase Antibody (PA5-87633) in WB
Western blot analysis of SERCA1 ATPase in C2C12 cells. Samples were incubated with SERCA1 ATPase Polyclonal antibody (Product # PA5-87633) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-87633
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 901-1001 of human SERCA1/SERCA1/ATP2A1 (NP_7752931)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.3 mg/mL
Amount
30 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HL-60; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane
Immunogen sequence: LSVLVTIEMC NALNSLSENQ SLLRMPPWVN IWLLGSICLS MSLHFLILYV DPLPMIFKLR ALDLTQWLMV LKISLPVIGL DEILKFVARN YLEDPEDERR K
Target Information
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; calcium pump 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; SERCA; SERCA ATPase; SR Ca(2+)-ATPase 1; unnamed protein product
Gene Aliases: ATP2A; SERCA1
UniProt ID: (Human) O14983, (Mouse) Q8R429
Entrez Gene ID: (Human) 487, (Mouse) 11937
calcium ion transport
cellular calcium ion homeostasis
regulation of muscle contraction
regulation of striated muscle contraction
apoptotic mitochondrial changes
organophosphate ester transport
nucleobase-containing compound transport
positive regulation of fast-twitch skeletal muscle fiber contraction
positive regulation of endoplasmic reticulum calcium ion concentration
negative regulation of endoplasmic reticulum calcium ion concentration
response to endoplasmic reticulum stress
response to peptide hormone
negative regulation of striated muscle contraction
positive regulation of mitochondrial calcium ion concentration
maintenance of mitochondrion location
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
sarcoplasmic reticulum calcium ion transport
calcium ion import
calcium ion transmembrane transport
relaxation of skeletal muscle
positive regulation of cardiac muscle cell contraction
carbohydrate derivative transport
positive regulation of calcium-transporting ATPase activity
positive regulation of calcium ion import into sarcoplasmic reticulum
calcium ion import into sarcoplasmic reticulum
ion transmembrane transport
regulation of cardiac conduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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